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METTL11B Antibody (C‑Terminus) LS‑C82571
METTL11B antibody LS-C82571 is an unconjugated rabbit polyclonal antibody to METTL11B from human, bat, dog and other species. Validated for WB.
Catalog
Size
Price
LS-C82571-50
50 µg
Unavailable

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Product Description

METTL11B antibody LS-C82571 is an unconjugated rabbit polyclonal antibody to METTL11B from human, bat, dog and other species. Validated for WB.
About METTL11B
Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes monomethylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and Pro in the Pro-Pro-Lys motif. May activate NTMT1 by priming its substrates for trimethylation. Q5VVY1 Q5VVY1

METTL11B Antibody, C1orf184 Antibody, Methyltransferase like 11B Antibody, HOMT1B Antibody, NTM1B Antibody


Specifications

Target
Human METTL11B
Host
Rabbit
Reactivity
Human, Bat, Dog, Horse (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Bovine, Hamster, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
METTL11B antibody was raised against synthetic peptide from C-Terminus of human METTL11B (Q5VVY1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Dog, Bat, Horse (100%); Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Rabbit (92%); Galago, Pig (85%); Guinea pig (84%).
Blocking Peptide
LS-E17799 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C82571. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human METTL11B
Usage
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/18/2018

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