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Anti-Melanoma Antigen NKI/C3 Antibody (clone NKI/C3) IHC-plus™ LS-B5564

Note: This antibody replaces LS-C134850

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

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Most Popular Melanoma Antigen NKI/C3 Antibodies

Anti-Melanoma Antigen NKI/C3 Antibody (clone NKI/C3) IHC-plus™ LS-B5564
Mouse Monoclonal [clone NKI/C3] (IgG1) to Human Melanoma Antigen NKI/C3
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone NKI/C3] (IgG1) to Human Melanoma Antigen NKI/C3
Human
IHC - Paraffin
Unconjugated

Details

Human Melanoma Antigen NKI/C3
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [NKI/C3]
Unconjugated
Ascites
Unmodified

Applications

  • IHC - Paraffin (1:100)

  • IHC - Frozen
  • Western blot

Specificity and Use

This antibody recognizes a heterogeneous 25-110 kD glycoprotein that is located mainly in the inner side of membranes of cytoplasmic vesicles in melanoma cells. The antigen has a 25 kD unglycosylated precursor in formalin fixed and paraffin-embedded tissue sections. Cross-reactivity: The antibody reacts with some mucus producing tumors, carcinoids, carcinomas of the thyroid, mast cells, histiocytes in tumor regions and with cells with secretory functions such as salivary glands, bronchial glands, sweat glands, pancreas and prostate.
The antibody should be used on formalin fixed paraffin embedded tissue sections, it is not advisable to use the antibody on frozen sections. Immunohistochemistry: Typical working dilution: 1:10-1:20. 60 minutes primary antibody incubation at 25 C. Standard ABC technique. Positive Control: Tonsil. Staining Pattern Cytoplasmic and membrane. Western Blot: Not recommended.

Packaging

Ascites, 50 mM PBS at, pH 7.4, 1% BSA, 15 mM sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About Melanoma Antigen NKI/C3


Immunohistochemistry

Immunohistochemistry
Anti-NKI/C3 antibody IHC of human intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5564 dilution 1:100.

Immunohistochemistry

Immunohistochemistry
Anti-NKI/C3 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5564 dilution 1:100.

Requested From: United States
Date Requested: 12/2/2016

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