Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone NKI/C3] (IgG1) to Human Melanoma Antigen NKI/C3
IHC - Paraffin
Human Melanoma Antigen NKI/C3
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [NKI/C3]
IHC - Paraffin (1:100)
IHC - Frozen
Specificity and Use
This antibody recognizes a heterogeneous 25-110 kD glycoprotein that is located mainly in the inner side of membranes of cytoplasmic vesicles in melanoma cells. The antigen has a 25 kD unglycosylated precursor in formalin fixed and paraffin-embedded tissue sections. Cross-reactivity: The antibody reacts with some mucus producing tumors, carcinoids, carcinomas of the thyroid, mast cells, histiocytes in tumor regions and with cells with secretory functions such as salivary glands, bronchial glands, sweat glands, pancreas and prostate.
The antibody should be used on formalin fixed paraffin embedded tissue sections, it is not advisable to use the antibody on frozen sections. Immunohistochemistry: Typical working dilution: 1:10-1:20. 60 minutes primary antibody incubation at 25 C. Standard ABC technique. Positive Control: Tonsil. Staining Pattern Cytoplasmic and membrane. Western Blot: Not recommended.
Ascites, 50 mM PBS at, pH 7.4, 1% BSA, 15 mM sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.