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Anti-MCM2 Antibody (clone CRCT2.1 (D1.9H5)) LS-C11595

Catalog Size Price
LS-C11595-100 100 µg (1 mg/ml) Unavailable

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100% Guaranteed
Mouse Monoclonal [clone CRCT2.1 (D1.9H5)] (IgG1) to Human MCM2
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IHC, IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated

Details

Human MCM2
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [CRCT2.1 (D1.9H5)]
Unconjugated
Tissue culture supernatant
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:200)
  • IHC - Frozen (1:500 - 1:1000)
  • Western blot

Specificity and Use

MCM2 antibody was raised against bacterially expressed human Mcm2.
Recognizes human DNA replication licensing factor MCM2, also known as Mcm2, Nuclear protein BM28 or mini chromosome maintenance protein-2. Mcm-2 is a 904 amino acid ~125kD nuclear protein involved in the control of DNA replication. Has been used successfully for the detection of human MCM2 in ovarian adenocarcinoma by immunohistochemistry on formalin fixed, paraffin embedded tissues.
Immunohistology: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections 0.01 M citrate buffer pH6 is recommended for this purpose.

Packaging

PBS, pH 7.4, 0.09% sodium azide.
For research use only.

About MCM2

P49736 NM_004526 NP_004517.2

MCM2 Antibody, BM28 Antibody, Cell devision cycle-like 1 Antibody, D3S3194 Antibody, Cdc19 Antibody, Cyclin-like 1 Antibody, KIAA0030 Antibody, Nuclear protein BM28 Antibody, CDCL1 Antibody, MITOTIN Antibody

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit.

Immunohistochemistry

Paraffin-embedded human tonsil stained with Mouse anti-Human MCM2 (LS-C11595) following citrate antigen retrieval.
Paraffin-embedded human tonsil stained with Mouse anti-Human MCM2 (LS-C11595) following citrate antigen retrieval.

Requested From: 
Date Requested: 5/24/2018

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