Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
Western blot (1:1000 - 1:5000)
ELISA (1:10000 - 1:100000)
An 18 aa peptide from the 1st extracellular domain of human MC3-R. The peptide was coupled with KLH.
1st Extracellular Domain
The 18 AA human MCR31 immunogenic peptide sequence is not very conserved in mouse (11/18 aa, 61%) or other species. It is recommended to use anti-mouse MCR32 that is made to the mouse sequence. Actual cross-reactivity of antibodies in all species is not established.
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure using ECL. ELISA: 1:10000-1:100000 using 50-100 ng of control peptide/well.
PBS, pH 7.2, 0.05% Sodium Azide
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year.
MC3R / MC3 Receptor is a G-protein-coupled receptor for melanocyte-stimulating hormone and adrenocorticotropic hormone that is expressed in tissues other than the adrenal cortex and melanocytes. This gene maps to the same region as the locus for benign neonatal epilepsy. Mice deficient for this gene have increased fat mass despite decreased food intake, suggesting a role for this gene product in the regulation of energy homeostasis.