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Anti-MBL2 / Mannose Binding Protein Antibody (clone 14D12) LS-C139870

Ordering

Wt. Vol. Conc. Price
100 µg - 0.1 mg/ml $595

LSBio (Direct) LSBio (Direct)
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MBL2 / Mannose Binding Protein Antibody LS-C139870 Images

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Immunohistochemistry
MBL-C (clone 14D12) deposition in developing murine atherosclerotic lesions following 10 weeks...

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Rat Monoclonal [clone 14D12] (IgG2a) to Mouse MBL2 / Mannose Binding Protein
Mouse
IHC - Frozen, Immunofluorescence, Western blot
Unconjugated

Details

Mouse MBL2 / Mannose Binding Protein
Rat
Mouse (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [14D12]
Purified

Applications

IHC - Frozen, Immunofluorescence, Western blot

Specificity and Use

MBL2 / Mannose Binding Protein antibody was raised against purified MBL-C emulsified in complete Freund's adjuvant
Mouse MBL-C / Mannose Binding Lectin C
For immuno histology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. It is recommended that solutions with a calcium concentration of 1 mM are used (14D12 is a calcium-dependent antibody).

Packaging

PBS, 0.02% sodium azide, 0.1% BSA
Store at 4°C, stable for one year.
For research use only.

About MBL2 / Mannose Binding Protein

P11226 NM_000242 NP_000233.1

MBL2 Antibody, Mannan-binding lectin Antibody, Mannan-binding protein Antibody, MBL2D Antibody, MBPD Antibody, MBL Antibody, Mannose-binding lectin Antibody, MBP-C Antibody, MBP1 Antibody, COLEC1 Antibody, Collectin-1 Antibody, HSMBPC Antibody, Mannose-binding protein C Antibody

Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

Immunohistochemistry

Immunohistochemistry
MBL-C (clone 14D12) deposition in developing murine atherosclerotic lesions following 10 weeks of high fat feeding. MBL-C was detected in and around invading macrophages invading the intima (insert). MBL-C bound, similar to MBL-A, at sites of necrosis (upper right corner). No MBL-C binding was shown in the media or on fibrous caps covering the thickened intima.
 

Requested From: United States
Date Requested: 8/27/2015

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