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Anti-MBD1 Antibody (clone 100B272.1.) LS-C563

Ordering

Wt. Vol. Conc. Price
100 µg 200 µl 0.5 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Mouse Monoclonal [clone 100B272.1.] (IgG1) to Human MBD1
Human, Monkey
Western blot
Unconjugated

Details

Human MBD1
Mouse
Human, Monkey (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [100B272.1.]
Protein G purified

Applications

Western blot (2 µg/ml)

Specificity and Use

MBD1 antibody was raised against this antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 391-405 (SESEDGAGSPPPYRR) of human MBD1; GenBank no ref|NP_056671.2|. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Marmoset, Tamarin (100%); Gibbon (93%); Horse (87%); Panda (80%).
There are many isoforms of human MBD1 ranging from 503 (~55kD) to 655 (~71kD) amino acids in length. Researchers are encouraged to use the provided immunogen sequence to determine if this is suitable for testing a specific isoform of interest.

Packaging

PBS, 0.02% sodium azide.
Long term: -70°C; Short term: +4°C
For research use only.

About MBD1

Q9UIS9 NM_015846 NP_056671.2

MBD1 Antibody, CXXC3 Antibody, RFT Antibody

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters.

Western blot

Western blot
Western blot of MBD1 in HeLa cell lysate using antibody at 2 ug/ml (lane 1) and 1 ug/ml (lane 2).
 

Requested From: United States
Date Requested: 9/25/2016

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