Target
Human MATN3 / Matrilin 3
Synonyms
MATN3 | DIPOA | EDM5 | HOA | Matrilin 3 | OADIP | OS2 | Matrilin-3
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
NS0-derived rhMatrilin-3.
Specificity
Selected for its ability to detect human Matrilin-3 in direct ELISAs and Western Blots. In these formats, this antibody shows 100% cross-reactivity with rmMatrilin-3 and no cross-reactivity with rhMatrilin-2 or rhMatrilin-4.
Usage
Western Blot: This antibody can be used at 1-2 ug/ml with the appropriate secondary reagents to detect human or mouse Matrilin-3. Using a colorimetric detection system, the detection limit for rhMatrilin-3 is approximately 1 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human or mouse Matrilin-3 The detection limit for rhMatrilin-3 is approximately 0.5 ng/well.
Presentation
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitution
Reconstitute in 200 ul sterile PBS
Storage
Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee