Human MARCH10

Goat

Human, Monkey, Rat (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-SRNYPQPRTEE, from the internal region of the protein sequence according to NP_001094345.1NP_689811.2.

aa778-788

Human MARCH10 / RNF190. This antibody is expected to recognise both reported isoforms (NP_001094345.1; NP_689811.2), which are identical.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (0.3 - 1 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:8000)

Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx. 90 kDa band observed in Rat Testis lysates (calculated MW of 90.5 kDa according to Human NP_689811.2 and of 87.9 kDa according to Mouse NP_766156.2). Recommended concentration: 0.3-1 ug/ml. An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunising peptide.

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q8NA82 NCBI: NM_152598 NP_689811.2