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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAPT / Tau Antibody (phospho‑Ser235) LS‑C203022
Tau antibody LS-C203022 is an unconjugated rabbit polyclonal antibody to Tau (MAPT) from human and mouse. Validated for ELISA and WB.
Catalog
Size
Price
LS-C203022-40
40 µg (0.5 mg/ml)
Unavailable

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Product Description

Tau antibody LS-C203022 is an unconjugated rabbit polyclonal antibody to Tau (MAPT) from human and mouse. Validated for ELISA and WB.
About MAPT / Tau
Tau promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. NM_016835

MAPT Antibody, FTDP-17 Antibody, MSTD Antibody, Neurofibrillary tangle protein Antibody, PHF-tau Antibody, MTBT2 Antibody, PPND Antibody, DDPAC Antibody, MAPTL Antibody, MTBT1 Antibody, Paired helical filament-tau Antibody, TAU Antibody


Specifications

Target
Human MAPT / Tau
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
MAPT / Tau antibody was raised against synthetic phospho-peptide (KLH-coupled) corresponding to the residues surrounding phospho-serine 235 of human tau
Epitope
pSer235
Specificity
Endogenous levels of tau protein only when phosphorylated at serine 235.
Presentation
Lyophilized from PBS, pH 7.4, 0.02% sodium azide.
Reconstitution
80 µl Distilled water
Storage
Store lyophilized at -20°C. The reconstituted product can be stored for short term at 4°C or long term at -20°C or below. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Western blot

Western blot analysis of mouse brain tissue lysate using 2 µg/ml Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody Lane 1: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody Lane 2: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with non-phospho-peptide Lane 3: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with phospho-peptide Lane 4: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with generic phospho-serine containing peptide The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG.
Western blot analysis of mouse brain tissue lysate using 2 µg/ml Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody Lane 1: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody Lane 2: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with non-phospho-peptide Lane 3: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with phospho-peptide Lane 4: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with generic phospho-serine containing peptide The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG.

Western blot

Western blot of mouse brain tissue lysate using 2 ug/ml Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody. Lane 1: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody. Lane 2: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with non-phospho-peptide. Lane 3: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with phospho-peptide. Lane 4: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with generic phospho-serine containing peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.
Western blot of mouse brain tissue lysate using 2 ug/ml Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody. Lane 1: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody. Lane 2: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with non-phospho-peptide. Lane 3: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with phospho-peptide. Lane 4: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with generic phospho-serine containing peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.

Western blot

Western blot analysis of mouse brain tissue lysate using 2 µg/ml Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody Lane 1: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody Lane 2: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with non-phospho-peptide Lane 3: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with phospho-peptide Lane 4: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with generic phospho-serine containing peptide The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG.
Western blot analysis of mouse brain tissue lysate using 2 µg/ml Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody Lane 1: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody Lane 2: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with non-phospho-peptide Lane 3: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with phospho-peptide Lane 4: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with generic phospho-serine containing peptide The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG.

Western blot

Western blot of mouse brain tissue lysate using 2 ug/ml Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody. Lane 1: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody. Lane 2: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with non-phospho-peptide. Lane 3: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with phospho-peptide. Lane 4: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with generic phospho-serine containing peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.
Western blot of mouse brain tissue lysate using 2 ug/ml Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody. Lane 1: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody. Lane 2: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with non-phospho-peptide. Lane 3: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with phospho-peptide. Lane 4: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with generic phospho-serine containing peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.

Western blot

Western blot analysis of mouse brain tissue lysate using 2 µg/ml Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody Lane 1: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody Lane 2: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with non-phospho-peptide Lane 3: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with phospho-peptide Lane 4: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with generic phospho-serine containing peptide The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG.
Western blot analysis of mouse brain tissue lysate using 2 µg/ml Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody Lane 1: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody Lane 2: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with non-phospho-peptide Lane 3: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with phospho-peptide Lane 4: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with generic phospho-serine containing peptide The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG.

Western blot

Western blot of mouse brain tissue lysate using 2 ug/ml Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody. Lane 1: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody. Lane 2: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with non-phospho-peptide. Lane 3: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with phospho-peptide. Lane 4: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with generic phospho-serine containing peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.
Western blot of mouse brain tissue lysate using 2 ug/ml Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody. Lane 1: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody. Lane 2: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with non-phospho-peptide. Lane 3: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with phospho-peptide. Lane 4: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with generic phospho-serine containing peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.

Western blot

Western blot analysis of mouse brain tissue lysate using 2 µg/ml Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody Lane 1: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody Lane 2: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with non-phospho-peptide Lane 3: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with phospho-peptide Lane 4: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with generic phospho-serine containing peptide The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG.
Western blot analysis of mouse brain tissue lysate using 2 µg/ml Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody Lane 1: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody Lane 2: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with non-phospho-peptide Lane 3: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with phospho-peptide Lane 4: Rabbit Anti-Tau (Phospho-Ser 235 ) Polyclonal Antibody pre-incubated with generic phospho-serine containing peptide The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG.

Western blot

Western blot of mouse brain tissue lysate using 2 ug/ml Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody. Lane 1: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody. Lane 2: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with non-phospho-peptide. Lane 3: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with phospho-peptide. Lane 4: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with generic phospho-serine containing peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.
Western blot of mouse brain tissue lysate using 2 ug/ml Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody. Lane 1: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody. Lane 2: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with non-phospho-peptide. Lane 3: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with phospho-peptide. Lane 4: Rabbit Anti-Tau (Phospho-Ser235) Polyclonal Antibody pre-incubated with generic phospho-serine containing peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.


Requested From: 
Date Requested: 6/21/2018

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