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Anti-MAPK6 / ERK3 Antibody (clone 1A9) LS-C114367

Catalog Size Price
LS-C114367-100 100 µl Unavailable

Related Products

46 MAPK6 / ERK3 Antibodies

Most Popular MAPK6 / ERK3 Antibodies

Anti-MAPK6 / ERK3 Antibody (aa461-510) IHC-plus™ LS-B9961
Rabbit Polyclonal (IgG) to Human MAPK6 / ERK3
Human, Mouse, Rat
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Anti-MAPK6 / ERK3 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9961 dilution 1:100.
Anti-MAPK6 / ERK3 Antibody (aa683-694) IHC-plus™ LS-B10395
Goat Polyclonal to Human MAPK6 / ERK3
Human, Mouse, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
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Anti-MAPK6 / ERK3 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10395 concentration 10 ug/ml.
Anti-MAPK6 / ERK3 Antibody (Internal) LS-C354065
Rabbit Polyclonal to Human MAPK6 / ERK3
Human, Monkey, Mouse, Bovine, Chicken
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
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Immunohistochemical analysis of ERK3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-MAPK6 / ERK3 Antibody (aa345-721, clone 5E1) LS-C355142
Mouse Monoclonal [clone 5E1] (IgG) to Human MAPK6 / ERK3
Human, Mouse
IHC, IHC - Paraffin, Immunofluorescence, Western blot
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IHC (P) using ERK3 / MAPK6 Antibody (5E1)
Anti-MAPK6 / ERK3 Antibody (aa520-721) LS-C407877
Rabbit Polyclonal to Human MAPK6 / ERK3
Human, Mouse, Rat, Dog
IHC, IHC - Paraffin, Western blot
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MAPK6 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.

100% Guaranteed
Mouse Monoclonal [clone 1A9] (IgG2a) to Human MAPK6 / ERK3
Human
IHC, IHC - Paraffin, Western blot
Unconjugated

Details

Human MAPK6 / ERK3
Mouse
IgG2a Monoclonal [1A9]
Unconjugated
Purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin
  • Western blot (1:1000)

Specificity and Use

MAPK6 / ERK3 antibody was raised against recombinant protein expressed in E. coli corresponding to amino acids 345-721 of human ERK3
Human MAPK6
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About MAPK6 / ERK3

Q16659 NM_002748 NP_002739.1

MAPK6 Antibody, ERK-3 Antibody, MAP kinase 6 Antibody, MAPK 6 Antibody, p97-MAPK Antibody, PRKM6 Antibody, MAP kinase isoform p97 Antibody, ERK3 Antibody, HsT17250 Antibody, p97MAPK Antibody

ERK3, a MAPK type protein kinase, has been associated with the ERK MAPKs based on similarity of the catalytic domain. Compared to ERK1/2, ERK 3 lacks the activating tyrosine phosphorylation site and contains only a serine which is phosphorylated by MEK. ERK3 is primarily localized to the nucleus where it is believed to phosphorylate substrates in response to cell stress and damage.

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded lung using anti-ERK3 mouse monoclonal antibody.
Immunohistochemical staining of paraffin-embedded lung using anti-ERK3 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.

Requested From: 
Date Requested: 5/25/2018

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