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Anti-MAP2K2 / MKK2 / MEK2 Antibody (phospho-Thr394) IHC-plus™ LS-B1727

Note: This antibody replaces LS-C34005, LS-C72370, LS-C51106, LS-C117414


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)

MAP2K2 / MKK2 / MEK2 Antibody LS-B1727 Images

[click image for a larger image and more details]
Anti-MAP2K2 / MEK2 antibody IHC of human skin.
Immunofluorescence staining of methanol-fixed HeLa cells using MEK-2(Phospho-Thr394) antibody.
Western blot
Western blot of extracts from HepG2 and HeLa cells untreated or treated with UV using...

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Rabbit Polyclonal (IgG) to Human MAP2K2 / MKK2 / MEK2
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot


Human MAP2K2 / MKK2 / MEK2
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


IHC - Paraffin (10 µg/ml), Immunofluorescence, Western blot

Specificity and Use

MAP2K2 / MKK2 / MEK2 antibody was raised against synthetic peptide from human MAP2K2 / MEK2.
Phosphopetide derived from human MEK2 around the phosphorylation site of threonine 394 (P-G-TP-P-T)
Immunohistochemistry: LS-B1727 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1727 was determined to be 10 ug/ml.


PBS, 0.02% sodium azide, 50% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About MAP2K2 / MKK2 / MEK2

P36507 NM_030662 NP_109587.1

MAP2K2 Antibody, ERK activator kinase 2 Antibody, MAP kinase kinase 2 Antibody, MAPK/ERK kinase 2 Antibody, MAPKK 2 Antibody, MKK2 Antibody, MEK 2 Antibody, MEK2 Antibody, MAPKK2 Antibody, PRKMK2 Antibody

MAP2K2 / MKK2 / MEK2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases.


Anti-MAP2K2 / MEK2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1727 concentration 10 ug/ml.


Immunofluorescence staining of methanol-fixed HeLa cells using MEK-2(Phospho-Thr394) antibody.

Western blot

Western blot
Western blot of extracts from HepG2 and HeLa cells untreated or treated with UV using MEK-2(Phospho-Thr394) antibody.

Requested From: United States
Date Requested: 10/13/2015

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