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Anti-MAP2K1 / MKK1 / MEK1 Antibody (phospho-Ser218/Ser222) LS-C25537


Wt. Vol. Conc. Price
200 µg - - $515
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular MAP2K1 / MKK1 / MEK1 Antibodies

Anti-MAP2K1 / MKK1 / MEK1 Antibody (aa264-313) IHC-plus™ LS-B7631
Rabbit Polyclonal (IgG) to Human MAP2K1 / MKK1 / MEK1
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-MAP2K1 / MKK1 / MEK1 Antibody (aa252-301) IHC-plus™ LS-B7632
Rabbit Polyclonal (IgG) to Human MAP2K1 / MKK1 / MEK1
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-MAP2K1 / MKK1 / MEK1 Antibody (Internal) LS-C356181
Rabbit Polyclonal to Human MAP2K1 / MKK1 / MEK1
Human, Monkey, Mouse, Rat, Bovine, Pig, Rabbit
IHC - Paraffin, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human MAP2K1 / MKK1 / MEK1
Human, Mouse, Rat
Western blot


Human MAP2K1 / MKK1 / MEK1
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Protein A purified


Western blot (1 µg/ml)

Specificity and Use

MAP2K1 / MKK1 / MEK1 antibody was raised against synthetic peptide corresponding to amino acids surrounding 218 and 222 of human MEK1 (KLH coupled). The immunizing sequence is highly conserved.
Recognizes MEK1/MEK2, Mr ~45kD. A non-specific protein was also detected in HEK293 cells, Mr ~50kD. Species cross-reactivity: Human, mouse and rat.
Suitable for use in Western Blot. Western Blot: 1 ug/ml detects phosphorylated MEK1/2 in RIPA lysates from HEK293, 3T3/A31 and PC-12 cells that had been treated with 5 Units of B-Raf for 30 minutes at 30?C. PC-12 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) (1 ug/ml). Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system.


0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About MAP2K1 / MKK1 / MEK1

Q02750 NM_002755 NP_002746.1

MAP2K1 Antibody, ERK activator kinase 1 Antibody, MAPK/ERK kinase 1 Antibody, MAPKK 1 Antibody, MKK1 Antibody, PRKMK1 Antibody, MEK 1 Antibody, MAP kinase kinase 1 Antibody, MAPKK1 Antibody, MEK1 Antibody

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2.

Requested From: United States
Date Requested: 10/22/2016

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