Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Bovine, Human, Rat, Xenopus
(tested or 100% immunogen sequence identity)
Protein G purified
IHC - Paraffin
Western blot (1 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
MAP2 antibody was raised against bovine brain MAP2. Cellular Localization: Cytoplasmic.
Recognizes the phosphorylated form of bovine MAP2a, b, c and shows no cross-reactivity with other MAPs, tau and tubulin. Recognizes MAP2a and MAP2b (~280kD doublet) and MAP2c (70kD). Its' epitope maps in the first 151 amino acids of MAP2. Reacts with dendrites and cell bodies of neurons. Species cross-reactivity: human, rat, Xenopus and quail.
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Western Blot: 1-2 ug/ml for 2 hrs at RT. Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes. followed by cooling at RT for 20 minutes. Positive control: IMR-5 or T98G human glioblastoma cells or brain.
PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
MAP2 is a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development.