MAP2 antibody LS-C16316 is an unconjugated mouse monoclonal antibody to MAP2 from human, rat, bovine and other species. Validated for IF, IHC and WB.
Bovine, Human, Rat, Xenopus
(tested or 100% immunogen sequence identity)
Protein G purified
- IHC - Paraffin
- Western blot (1 - 2 µg/ml)
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MAP2 antibody was raised against bovine brain MAP2. Cellular Localization: Cytoplasmic.
Recognizes the phosphorylated form of bovine MAP2a, b, c and shows no cross-reactivity with other MAPs, tau and tubulin. Recognizes MAP2a and MAP2b (~280kD doublet) and MAP2c (70kD). Its' epitope maps in the first 151 amino acids of MAP2. Reacts with dendrites and cell bodies of neurons. Species cross-reactivity: human, rat, Xenopus and quail.
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Western Blot: 1-2 ug/ml for 2 hrs at RT. Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes. followed by cooling at RT for 20 minutes. Positive control: IMR-5 or T98G human glioblastoma cells or brain.
PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.