Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 4E7] (IgG1) to Human Mannose Phosphate Isomerase
IHC - Paraffin, Western blot, Flow Cytometry
Rabbit Polyclonal (IgG) to Human Mannose Phosphate Isomerase
Human Mannose Phosphate Isomerase
Human (tested or 100% immunogen sequence identity)
Mouse, Rat, Bovine (at least 90% immunogen sequence identity)
Western blot (1:500 - 1:3000)
Specificity and Use
Mannose Phosphate Isomerase antibody was raised against recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 154 (P34949) of MPI. Percent identity by BLAST analysis: Human (100%); Bovine (95%); Rat (91%); Mouse (90%).
Human MPI / PMI1
0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants.