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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAGED1 / NRAGE Antibody (clone 1E1) LS‑C134002
NRAGE antibody LS-C134002 is an unconjugated mouse monoclonal antibody to human NRAGE (MAGED1). Validated for ELISA and WB.
Catalog
Size
Price
LS-C134002-100
100 µg (0.36 mg/ml)
Unavailable

Popular MAGED1 / NRAGE Products

Antibody
Species: Rat, Human, Mouse
Applications: IHC, Western blot, Immunoprecipitation
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry of paraffin-embedded liver cancer tissue.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Immunohistochemistry of Human thyroid cancer using MAGED1 Polyclonal Antibody at dilution of 1:30.
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

NRAGE antibody LS-C134002 is an unconjugated mouse monoclonal antibody to human NRAGE (MAGED1). Validated for ELISA and WB.
About MAGED1 / NRAGE
Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Inhibits cell cycle progression, and facilitates NGFR-mediated apoptosis. May act as a regulator of the function of DLX family members. May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. NM_006986 NP_008917.3

MAGED1 Antibody, DLXIN-1 Antibody, MAGE-D1 antigen Antibody, Melanoma antigen family D, 1 Antibody, NRAGE Antibody, MAGE tumor antigen CCF Antibody, Melanoma-associated antigen D1 Antibody


Specifications

Target
Human MAGED1 / NRAGE
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal [1E1]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
MAGED1 / NRAGE antibody was raised against mAGED1 (NP_001005333, 117 a.a. ~ 227 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human MAGED1
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

MAGED1 monoclonal antibody (M06), clone 1E1. Western blot of MAGED1 expression in Jurkat.
MAGED1 monoclonal antibody (M06), clone 1E1. Western blot of MAGED1 expression in Jurkat.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged MAGED1 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged MAGED1 is approximately 1 ng/ml as a capture antibody.

Western blot

MAGED1 monoclonal antibody (M06), clone 1E1. Western blot of MAGED1 expression in Jurkat.
MAGED1 monoclonal antibody (M06), clone 1E1. Western blot of MAGED1 expression in Jurkat.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged MAGED1 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged MAGED1 is approximately 1 ng/ml as a capture antibody.

Western blot

MAGED1 monoclonal antibody (M06), clone 1E1. Western blot of MAGED1 expression in Jurkat.
MAGED1 monoclonal antibody (M06), clone 1E1. Western blot of MAGED1 expression in Jurkat.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged MAGED1 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged MAGED1 is approximately 1 ng/ml as a capture antibody.

Western blot

MAGED1 monoclonal antibody (M06), clone 1E1. Western blot of MAGED1 expression in Jurkat.
MAGED1 monoclonal antibody (M06), clone 1E1. Western blot of MAGED1 expression in Jurkat.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged MAGED1 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged MAGED1 is approximately 1 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/24/2018

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