Macrophage

Mouse

Human (tested or 100% immunogen sequence identity)

Monoclonal

25F9

Biotin

Affinity purified

Unmodified

Cultured human monocytes

The antigen is a 86kDa membrane protein, the epitope has not been further characterized. Antigen distribution: Isolated cells: Absent on freshly isolated monocytes and other blood cells; present on 40 - 50% of human monocytes after 6-7 days in culture, also positive on some melanoma and carcinoma cell lines. Tissue sections: Kupffer cells, histiocytes (skin), macrophages of the thymus, in the germinal centres of lymph nodes and spleen, in mamma carcinoma, melanoma, osteocarcinoma and gastric cancer; eczema, sarcoidosis, BCG granuloma; synovial lining cells, tuberculoid leprosy: no expression in lepromatous leprosy. Specificity: Human: mature macrophages and monocytes. Other: subpopulation of macrophages in Rhesus monkey; reactive with pig alveolar macrophages and Kupffer cells.

• IHC
• IHC - Paraffin (1:200)

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from PBS pH 7.2, 10mg/ml BSA, 0.01% thimerosal

Reconstitute with 0.5ml distilled H2O.

Store at 4°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.