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Anti-Macrophage Antibody (clone 25F9, Biotin) LS-C106526


Wt. Vol. Conc. Price
25 µg - - $265
100 µg - - $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

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100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 25F9] (IgG1) to Human Macrophage
Flow Cytometry
Biotin Conjugated


Human Macrophage
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [25F9]
Affinity purified


Flow Cytometry

Specificity and Use

Macrophage antibody was raised against human Macrophage, Late Stage Inflammatory
This 25F9 (25F9) antibody has been tested by flow cytometric analysis of cultured human macrophages. This can be used at less than or equal to 0.25 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.


PBS, pH 7.2, <=0.09% sodium azide
Store at 4°C. Do not freeze.
For research use only.

About Macrophage

Macrophages (Greek: big eaters, from makros "large" + phagein "eat"; abbr. MF), are a type of white blood cell that engulfs and digests cellular debris, foreign substances, microbes, cancer cells, and anything else that does not have the types of proteins specific to the surface of healthy body cells on its surface in a process called phagocytosis. Macrophages were first discovered by Élie Metchnikoff, a Russian bacteriologist, in 1884.

Flow Cytometry

Flow Cytometry
Staining of cultured macrophages from peripheral blood monocytes with 0.125 ug of Biotin Mouse IgG1, K isotype control (blue histogram) or 0.125 ug of Biotin anti-human mature macrophage marker (25F9) (purple histogram) followed by Streptavidin-PE. Total viable cells were used for analysis.

Requested From: United States
Date Requested: 10/22/2016

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