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Anti-LRP5 Antibody (C-Terminus) LS-C8514

Ordering

Wt. Vol. Conc. Price
50 µg 200 µl - $385
100 µg - - $530
Inquire for larger quantities

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206-374-1102
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Rabbit Polyclonal (IgG) to Human LRP5
Human, Mouse
Immunofluorescence, Western blot
Unconjugated

Details

Human LRP5
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

Immunofluorescence (2 - 3 µg/ml), Western blot (1 - 3 µg/ml)

Specificity and Use

LRP5 antibody was raised against synthetic peptide derived from the C-terminal region of the human LRP5 protein. This intracellular region is identical to the corresponding mouse and rat LRP5 amino acid sequences.
C-Terminus
Recognizes human LRP5 protein. Western Blot tests using negative control mock pSEC-tag-transfected 293T cell lysates did not yield the target band. Species cross-reactivity: Mouse.
Suitable for use in Immunofluorescence and Western Blot. Immunofluorescence: 2-3 ug/ml; Note: For optimal results in immunofluorescence assays, cells should be fixed in 4% paraformaldehyde/0.5% Triton X-100/PBS for 10 minutes, permeabilized with 0.5% Triton X-100/PBS for 5 minutes, and blocked with 5% BSA/PBS for 30 minutes. Western Blot: 1-3 ug/ml; Single band at ~200kD. Positive control: Mouse LRP5-pSEC-tag-transfected 293T cells, MCF-7 human breast adenocarcinoma, T47D human breast ductal carcinoma, and NIH-3T3 mouse embryo fibroblast lysates.

Packaging

PBS, pH 7.4, 0.1% sodium azide, 40% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About LRP5

O75197 NM_002335 NP_002326.2

LRP5 Antibody, EVR1 Antibody, Exudative vitreoretinopathy 1 Antibody, LRP7 Antibody, OPTA1 Antibody, OPPG Antibody, OPS Antibody, LR3 Antibody, LRP-5 Antibody, VBCH2 Antibody, BMND1 Antibody, EVR4 Antibody

Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation.

Requested From: United States
Date Requested: 9/27/2016

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