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Anti-LPL / Lipoprotein Lipase Antibody LS-C150114

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular LPL / Lipoprotein Lipase Antibodies

Anti-LPL / Lipoprotein Lipase Antibody (clone Mab-7) LS-C85283
Mouse Monoclonal [clone Mab-7] (IgG1) to Bovine LPL / Lipoprotein Lipase
Bovine
Unconjugated
Anti-LPL / Lipoprotein Lipase Antibody (clone 5D2, Biotin) LS-C188627
Mouse Monoclonal [clone 5D2] (IgG1) to Bovine LPL / Lipoprotein Lipase
Bovine, Human, Rat, Guinea pig, Pig, Chicken
Western blot, Immunoprecipitation, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human LPL / Lipoprotein Lipase
Human, Mouse
ICC, Western blot
Unconjugated

Details

Human LPL / Lipoprotein Lipase
Goat
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (5 - 15 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

LPL / Lipoprotein Lipase antibody was raised against e. coli-derived recombinant human Lipoprotein Lipase/LPL Accession # P06858.
Detects recombinant human Lipoprotein Lipase/LPL in direct ELISAs and Western blots. Detects human and mouse Lipoprotein Lipase/LPL in.

Packaging

Lyophilized from 0.2 µm filtered PBS, pH 7.4, 5% trehalose
Sterile PBS. Resuspend to 500 ul
Store at -20 to -70 C. Stable for 12 months from date of receipt.
For research use only.

About LPL / Lipoprotein Lipase

P06858 NM_000237 NP_000228.1

LPL Antibody, LIPD Antibody, Lipoprotein lipase Antibody, HDLCQ11 Antibody

The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium.

Immunocytochemistry

Immunocytochemistry
Lipoprotein Lipase/LPL in SH-SY5Y Human Cell Line. Lipoprotein Lipase/LPL was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Goat Anti-Human Lipoprotein Lipase/LPL Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. This application has not been tested in mouse samples.

Western blot

Western blot
Detection of Human and Mouse Lipoprotein Lipase/LPL by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and NMuMG mouse mammary gland epithelial cell line. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human Lipoprotein Lipase/LPL Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Lipoprotein Lipase/LPL at approximately 56 kD (as indicated). This experiment was conducted under reducing conditions.

Requested From: United States
Date Requested: 12/8/2016

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