LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-LPIN3 / Lipin-3 Antibody (aa428-529) LS-C37207

Ordering

Wt. Vol. Conc. Price
100 µg 500 µl 0.2 mg/ml $635
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular LPIN3 / Lipin-3 Antibodies

Anti-LPIN3 / Lipin-3 Antibody (aa428-529) LS-C37207
Goat Polyclonal (IgG) to Mouse LPIN3 / Lipin-3
Mouse
IHC, ICC, Western blot, ELISA
Unconjugated
Anti-LPIN3 / Lipin-3 Antibody (aa149-160) IHC-plus™ LS-B2499
Goat Polyclonal to Human LPIN3 / Lipin-3
Human, Monkey
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-LPIN3 / Lipin-3 Antibody (clone 3H3) LS-C339410
Mouse Monoclonal [clone 3H3] (IgG1) to Human LPIN3 / Lipin-3
Human
Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Mouse LPIN3 / Lipin-3
Mouse
IHC, ICC, Western blot, ELISA
Unconjugated

Details

Mouse LPIN3 / Lipin-3
Goat
Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC (10 µg/ml)
  • ICC (10 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 1 µg/ml)

Specificity and Use

LPIN3 / Lipin-3 antibody was raised against e. coli-derived recombinant mouse Lipin 3 (aa428-529).
aa428-529
Selected for its ability to recognize Lipin 3 in the applications listed. In direct ELISAs and Western Blots, this antibody shows ~50% cross-reactivity with rhLipin 3 and less than 5% cross-reactivity with rhLipin 1 and rhLipin 2.
Suitable for use in ELISA, Western Blot, Immunocytochemistry. Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect mouse rmLipin 3. The detection limit for rmLipin 3 is approximately 2 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/ml to detect Lipin 3 on CCD-18co normal human colon fibroblasts. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4?C followed by donkey anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Immunohistochemistry: 10 ug/ml to detect Lipin 3 in cryosections of adult mouse intestine. Tissue was fixed overnight in 4% PFA + 14% picric acid in PBS at 4?C. Sections were blocked with 10% donkey serum and 0.1% Triton in 1% BSA in PBS for 45 minutes at RT. After blocking, sections were incubated with primary antibody for 3 hours at RT followed by donkey anti-goat lgG at RT for 1 hour. Between each step, sections were washed with 0.1% BSA in PBS. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect mouse rmLipin 3. The detection limit for rmLipin 3 is approximately 0.05 ng/well.

Packaging

Lyophilized from PBS, 5% trehalose
500 µl Sterile PBS.
Lyophilized powder may be stored at -20°C for short-term only. Reconstituted product is stable for 12 months at -20°C.
For research use only.

About LPIN3 / Lipin-3

Q9BQK8 NP_075047.1

LPIN3 Antibody, DJ620E11.2 Antibody, DJ450M14.2 Antibody, Lipin-3 Antibody, Lipin 3 Antibody, Lipin 3-like Antibody, Lipin-3-like Antibody, SMP2 Antibody, DJ450M14.3 Antibody, LIPN3L Antibody

LPIN3 / Lipin-3 is a member of the lipin family of proteins, and all family members share strong homology in their C-terminal region. This protein is thought to form hetero-oligomers with other lipin family members, while one family member, lipin 1, can also form homo-oligomers. This protein contains conserved motifs for phosphatidate phosphatase 1 (PAP1) activity as well as a domain that interacts with a transcriptional co-activator.

Requested From: United States
Date Requested: 12/10/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number