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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LPIN3 / Lipin‑3 Antibody (aa428‑529) LS‑C37207 Best Seller
Lipin-3 antibody LS-C37207 is an unconjugated goat polyclonal antibody to mouse Lipin-3 (LPIN3). Validated for ELISA, ICC, IHC and WB. Cited in 2 publications.
Antibody
Catalog
Size
Price
LS-C37207-100
100 µg (0.2 mg/ml)
$725

Popular LPIN3 / Lipin-3 Products

Antibody
Species: Mouse
Applications: Western blot, ELISA
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LPIN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LPIN3.
Species: Human
Applications: Western blot
Anti-LPIN3 / Lipin 3 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2499 concentration 5 ug/ml.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
IHC of paraffin-embedded Human endometrium tissue using anti-LPIN3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

Lipin-3 antibody LS-C37207 is an unconjugated goat polyclonal antibody to mouse Lipin-3 (LPIN3). Validated for ELISA, ICC, IHC and WB. Cited in 2 publications.
About LPIN3 / Lipin-3
LPIN3 / Lipin-3 is a member of the lipin family of proteins, and all family members share strong homology in their C-terminal region. This protein is thought to form hetero-oligomers with other lipin family members, while one family member, lipin 1, can also form homo-oligomers. This protein contains conserved motifs for phosphatidate phosphatase 1 (PAP1) activity as well as a domain that interacts with a transcriptional co-activator. Q9BQK8 NP_075047.1

LPIN3 Antibody, DJ620E11.2 Antibody, DJ450M14.2 Antibody, Lipin-3 Antibody, Lipin 3 Antibody, Lipin 3-like Antibody, Lipin-3-like Antibody, SMP2 Antibody, DJ450M14.3 Antibody, LIPN3L Antibody


Specifications

Target
Mouse LPIN3 / Lipin-3
Host
Goat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (10 µg/ml)
  • ICC (10 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
LPIN3 / Lipin-3 antibody was raised against e. coli-derived recombinant mouse Lipin 3 (aa428-529).
Epitope
aa428-529
Specificity
Selected for its ability to recognize Lipin 3 in the applications listed. In direct ELISAs and Western Blots, this antibody shows ~50% cross-reactivity with rhLipin 3 and less than 5% cross-reactivity with rhLipin 1 and rhLipin 2.
Usage
Suitable for use in ELISA, Western Blot, Immunocytochemistry. Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect mouse rmLipin 3. The detection limit for rmLipin 3 is approximately 2 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/ml to detect Lipin 3 on CCD-18co normal human colon fibroblasts. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4?C followed by donkey anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Immunohistochemistry: 10 ug/ml to detect Lipin 3 in cryosections of adult mouse intestine. Tissue was fixed overnight in 4% PFA + 14% picric acid in PBS at 4?C. Sections were blocked with 10% donkey serum and 0.1% Triton in 1% BSA in PBS for 45 minutes at RT. After blocking, sections were incubated with primary antibody for 3 hours at RT followed by donkey anti-goat lgG at RT for 1 hour. Between each step, sections were washed with 0.1% BSA in PBS. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect mouse rmLipin 3. The detection limit for rmLipin 3 is approximately 0.05 ng/well.
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
500 µl Sterile PBS.
Storage
Lyophilized powder may be stored at -20°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
Restrictions
For research use only.

Publications (2)

1
Lipin-1 and lipin-3 together determine adiposity in vivo. Csaki LS, Dwyer JR, Li X, Nguyen MH, Dewald J, Brindley DN, Lusis AJ, Yoshinaga Y, de Jong P, Fong L, Young SG, Reue K. Molecular metabolism. 2014 3:145-54. (ICC; Mouse) [Full Text Article] [PubMed:24634820]
2
Mouse lipin-1 and lipin-2 cooperate to maintain glycerolipid homeostasis in liver and aging cerebellum. Dwyer JR, Donkor J, Zhang P, Csaki LS, Vergnes L, Lee JM, Dewald J, Brindley DN, Atti E, Tetradis S, Yoshinaga Y, De Jong PJ, Fong LG, Young SG, Reue K. Proceedings of the National Academy of Sciences of the United States of America. 2012 109:E2486-95. (ICC; Mouse) [Full Text Article] [PubMed:22908270]

Customer Reviews (0)


Requested From: United States
Date Requested: 9/25/2018

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