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Anti-LIN28B Antibody (aa1-30) IHC-plus™ LS-B3423

Note: This antibody replaces LS-C98240

Ordering

Wt. Vol. Conc. Price
- 200 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular LIN28B Antibodies

Anti-LIN28B Antibody (aa1-30) IHC-plus™ LS-B3423
Rabbit Polyclonal to Human LIN28B
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-LIN28B Antibody (aa6-36, Biotin) LS-C237139
Rabbit Polyclonal (IgG) to Human LIN28B
Human
IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human LIN28B
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human LIN28B
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

LIN28B antibody was raised against kLH-conjugated synthetic peptide between amino acids 1-30 from N-terminal region of human LIN28B.
LS-E3657 - Lyophilized - 100 µg - $145.00
aa1-30
Human LIN28B
Immunohistochemistry: LS-B3423 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3423 was determined to be 10 ug/ml.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

Publications (1)

1
Lin28A and Lin28B inhibit let-7 microRNA biogenesis by distinct mechanisms. Piskounova E, Polytarchou C, Thornton JE, LaPierre RJ, Pothoulakis C, Hagan JP, Iliopoulos D, Gregory RI. Cell. 2011 Nov;147:1066-79. [Full Text Article] [PubMed:22118463] [PMC:PMC3227872]

About LIN28B

Q6ZN17 NP_001004317.1

LIN28B Antibody, CSDD2 Antibody, Lin-28B Antibody, Lin-28 homolog B (C. elegans) Antibody, Protein lin-28 homolog B Antibody, Lin-28.2 Antibody

Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7.

Immunohistochemistry

Immunohistochemistry
Anti-LIN28B antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3423 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-LIN28B antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3423 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of SY5Y cells stained with LIN28B antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated LIN28B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the LIN28B immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000187772).

Western blot

Western blot
Western blot of LIN28B Antibody in HL60 cell line lysates (35 ug/lane). LIN28B (arrow) was detected using the purified antibody.

Flow Cytometry

Flow Cytometry
Flow cytometric of HL-60 cells using LIN28B Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
 

Requested From: United States
Date Requested: 12/10/2016

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