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Anti-LFNG / Lunatic Fringe Antibody (aa74-123) IHC-plus™ LS-B9718

Note: This antibody replaces LS-C80683

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular LFNG / Lunatic Fringe Antibodies

Anti-LFNG / Lunatic Fringe Antibody (aa74-123) IHC-plus™ LS-B9718
Rabbit Polyclonal (IgG) to Human LFNG / Lunatic Fringe
Human, Monkey, Mouse, Rat, Guinea pig, Hamster, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-LFNG / Lunatic Fringe Antibody (aa86-114) IHC-plus™ LS-B10951
Rabbit Polyclonal to Human LFNG / Lunatic Fringe
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human LFNG / Lunatic Fringe
Human, Monkey, Mouse, Rat, Guinea pig, Hamster, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human LFNG / Lunatic Fringe
Rabbit
Human, Monkey, Mouse, Rat, Guinea pig, Hamster, Rabbit (tested or 100% immunogen sequence identity)
Bovine (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

LFNG / Lunatic Fringe antibody was raised against synthetic peptide located between aa74-123 of human LFNG (Q8NES3-3, NP_001035258). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Guinea pig (100%); Gibbon, Galago, Bovine (92%); Rabbit (84%).
aa74-123
Human LFNG
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About LFNG / Lunatic Fringe

Q8NES3 U94354 AAC51360.1

LFNG Antibody, SCDO3 Antibody

LFNG / Lunatic Fringe is a member of the fringe gene family which also includes radical and manic fringe genes. They all encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development.

Immunohistochemistry

Immunohistochemistry
Anti-LFNG / Lunatic Fringe antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9718 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/9/2016

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