Human LEF1

Rabbit

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

IgG Monoclonal

Unconjugated

Tissue culture supernatant

Unmodified

Synthetic peptide (KLH-coupled) corresponding to residues surrounding Pro82 of human LEF1.

Pro82

Detects endogenous level of total human LEF1 protein. Does not recognize the dominant negative forms of LEF1 generated by an alternative promoter. Species cross-reactivity: Mouse and rat.

• ICC
• Immunofluorescence
• Western blot (1:1000)
• Immunoprecipitation (1:50)
• Flow Cytometry (1:200)

Suitable for use in Western Blot, Immunoprecipitation, Flow Cytometry, Immunofluorescence and Immunocytochemistry. Other applications not tested.Recommended Dilution:Western Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4ºC with gentle shaking, overnight.Immunoprecipitation: 1:50Flow Cytometry: 1:200Immunofluorescence (IF-IC): 1:100Optimal dilutions to be determined by the researcher.

10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 100 ug/ml BSA, 0.02% Sodium Azide, 50% Glycerol

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9UJU2 NCBI: NM_016269 NP_057353.1