Target
Human LEF1
Synonyms
LEF1 | LEF-1 | TCF7L3 | TCF1-alpha | TCF10 | TCF1ALPHA
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Immunogen
Synthetic peptide (KLH-coupled) corresponding to residues surrounding Pro82 of human LEF1.
Specificity
Detects endogenous level of total human LEF1 protein. Does not recognize the dominant negative forms of LEF1 generated by an alternative promoter. Species cross-reactivity: Mouse and rat.
Applications
- ICC
- Immunofluorescence
- Western blot (1:1000)
- Immunoprecipitation (1:50)
- Flow Cytometry (1:200)
Usage
Suitable for use in Western Blot, Immunoprecipitation, Flow Cytometry, Immunofluorescence and Immunocytochemistry. Other applications not tested.Recommended Dilution:Western Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4ºC with gentle shaking, overnight.Immunoprecipitation: 1:50Flow Cytometry: 1:200Immunofluorescence (IF-IC): 1:100Optimal dilutions to be determined by the researcher.
Presentation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 100 ug/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee