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Anti-LacI / Lac Repressor Antibody LS-C60143


Wt. Vol. Conc. Price
100 µg - 1 mg/ml Unavailable

Most Popular LacI / Lac Repressor Antibodies

Anti-LacI / Lac Repressor Antibody LS-C60143
Rabbit Polyclonal to E. coli LacI / Lac Repressor
E. coli, S. sonnei
Western blot, ELISA
Western blot Image

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Rabbit Polyclonal to E. coli LacI / Lac Repressor
E. coli, S. sonnei
Western blot, ELISA


E. coli LacI / Lac Repressor
E. coli, S. sonnei (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • Western blot (1:500 - 1:2000)
  • ELISA (1:90000 - 1:100000)

Specificity and Use

Recombinant Lac I protein.
recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100% homology with the immunizing sequence. Cross-reactivity with Lac I from other sources has not been determined
This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Lac I protein. Specific conditions for reactivity and detection of Lac I should be optimized by the end user. Expect a band approximately ~38 kD in size corresponding to Lac I by western blotting in the appropriate cell lysate or extract.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store at -20°C. Aliquot to avoid freeze/thaw cycles. Do not freeze diluted antibody.
For research use only.

About LacI / Lac Repressor

Western blot

Western blot
Anti-Lac I Antibody - Western Blot. Western blot of affinity purified anti-Lac I antibody shows detection of a 38 kD band corresponding to recombinant Lac I (arrowhead). The blot was blocked with 5% BLOTTO in TTBS overnight at 4C. Primary antibody was used at a 1:1000 dilution followed by reaction with a 1:10000 dilution of HRP goat anti-rabbit IgG. ECL was used for detection. Personal communication, S. Hughes & M. Abram, NCI, Bethesda, MD.

Requested From: 
Date Requested: 4/26/2017

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