Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
E. coli, S. sonnei (tested or 100% immunogen sequence identity)
Western blot (1:500 - 1:2000)
ELISA (1:90000 - 1:100000)
Specificity and Use
Recombinant Lac I protein.
recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100% homology with the immunizing sequence. Cross-reactivity with Lac I from other sources has not been determined
This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Lac I protein. Specific conditions for reactivity and detection of Lac I should be optimized by the end user. Expect a band approximately ~38 kD in size corresponding to Lac I by western blotting in the appropriate cell lysate or extract.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store at -20°C. Aliquot to avoid freeze/thaw cycles. Do not freeze diluted antibody.
Anti-Lac I Antibody - Western Blot. Western blot of affinity purified anti-Lac I antibody shows detection of a 38 kD band corresponding to recombinant Lac I (arrowhead). The blot was blocked with 5% BLOTTO in TTBS overnight at 4C. Primary antibody was used at a 1:1000 dilution followed by reaction with a 1:10000 dilution of HRP goat anti-rabbit IgG. ECL was used for detection. Personal communication, S. Hughes & M. Abram, NCI, Bethesda, MD.