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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KHK / Ketohexokinase Antibody (clone 1D8) LS‑C115290
Ketohexokinase antibody LS-C115290 is an unconjugated mouse monoclonal antibody to human Ketohexokinase (KHK). Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C115290-100
100 µl
Unavailable

Popular KHK / Ketohexokinase Products

Western blot of KHK (arrow) using rabbit polyclonal Ketohexokinase (KHK) Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KHK gene (Lane 2) (Origene Technologies).
Species: Human, Mouse
Applications: Western blot
IHC of paraffin-embedded Human liver tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-KHK mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

Ketohexokinase antibody LS-C115290 is an unconjugated mouse monoclonal antibody to human Ketohexokinase (KHK). Validated for Flow, IF, IHC and WB.
About KHK / Ketohexokinase
KHK / Ketohexokinase encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. P50053 NM_000221 NP_000212.1

KHK Antibody, Ketohexokinase (fructokinase) Antibody, Hepatic fructokinase Antibody, Ketohexokinase Antibody


Specifications

Target
Human KHK / Ketohexokinase
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [1D8]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Full length human recombinant protein of human KHK (NP_000212) produced in HEK293T cell.
Specificity
Human KHK / Ketohexokinase
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human thyroid tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human thyroid tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human Kidney tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human liver tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human liver tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-KHK mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KHK.
Anti-KHK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KHK.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KHK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KHK.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KHK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KHK.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KHK antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KHK antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human thyroid tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human thyroid tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human Kidney tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human liver tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human liver tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-KHK mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KHK.
Anti-KHK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KHK.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KHK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KHK.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KHK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KHK.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KHK antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KHK antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human thyroid tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human thyroid tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human Kidney tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human liver tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human liver tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-KHK mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KHK.
Anti-KHK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KHK.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KHK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KHK.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KHK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KHK.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KHK antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KHK antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human thyroid tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human thyroid tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human Kidney tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Human liver tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Human liver tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KHK mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-KHK mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KHK.
Anti-KHK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KHK.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KHK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KHK.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KHK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KHK.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KHK antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KHK antibody, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/23/2018

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