Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone EFG-11] (IgG2b) to Human Keratan Sulfate
Human, Mouse, Sheep
Western blot, ELISA, Functional Assay, Radioimmunoassay
Human Keratan Sulfate
Human, Mouse, Sheep (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [EFG-11]
Western blot (1:500 - 1:2000)
ELISA (1:2500 - 1:10000)
Radioimmunoassay (1:15000 - 1:250000)
Specificity and Use
Keratan Sulfate antibody was raised against human articular cartilage proteoglycan digested with chondroitinase ABC.
Recognizes 2-3 disaccharide units on both skeletal (type II) and corneal (type I) keratan sulfate chains either in isolated or proteoglycan-bound form. The epitope is resistant to proteolytic or alkaline cleavages and is partially sensitive to keratanase or other endo-b-galactosidase treatment. Species cross-reactivity: Human, bovine, sheep, porcine, canine, mouse (adult). No cross-reactivity with chicken limb bud proteoglycans, dermatan sulfate proteoglycans of cartilage and proteoglycans of rat chondrosarcomas.
Suitable for use in Western Blot, ELISA and RIA. May be used in a competitive inhibition assay to measure keratan sulfate levels. Western Blot: 1:500-1:2000. ELISA: 1:2500-1:10000. RIA:1:15000-1:250000.
Supplied as a liquid. No preservatives added.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.