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KCNC3 / Kv3.3 Antibody (aa651‑663) LS‑C154944
Kv3.3 antibody LS-C154944 is an unconjugated goat polyclonal antibody to Kv3.3 (KCNC3) from mouse and rat. Validated for Peptide-ELISA and WB.
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Species:
Human, Monkey, Mouse, Rat, Guinea pig, Pig, Rabbit
Applications:
Western blot
![Immunofluorescence of monoclonal antibody to KCNC3 on NIH/3T3 cell. [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com//image2/kcnc3-kv3.3-antibody-clone-1c1-ls-c197570/145477_1111985.jpg)
Species:
Human, Mouse
Applications:
Immunofluorescence, Western blot, ELISA
Product Description
Kv3.3 antibody LS-C154944 is an unconjugated goat polyclonal antibody to Kv3.3 (KCNC3) from mouse and rat. Validated for Peptide-ELISA and WB.
About KCNC3 / Kv3.3
This protein mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
Q14003
NM_004977
NP_004968.2
KCNC3 Antibody, KSHIIID Antibody, SCA13 Antibody, Spinocerebellar ataxia 13 Antibody, KV3.3 Antibody
Specifications
Target
Mouse KCNC3 / Kv3.3
Host
Goat
Reactivity
Mouse, Rat
(tested or 100% immunogen sequence identity)
Predicted
Human, Monkey, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- Western blot (0.2 - 0.6 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Immunogen
KCNC3 / Kv3.3 antibody was raised against synthetic peptide C-QEEVIETNRADPR from an internal region of mouse KCNC3 / Kv3.3 (NP_032448.2). Percent identity by BLAST analysis: Mouse, Rat (100%); Human, Orangutan, Gibbon, Monkey, Galago, Marmoset, Panda, Rabbit, Porcine (92%); Guinea pig (85%).
Blocking Peptide
LS-E27809 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C154944. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-C154944. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa651-663
Specificity
Mouse KCNC3 / Kv3.3.
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 80kD band observed in Mouse fetal Brain lysates (calculated MW of 81.9kD according to NP_032448.2). Recommended concentration: 0.2-0.6 ug/ml. Some minor background is detected and is blocked by the immunizing peptide. We call for caution when used for other assays than Western blot.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Western blot
KCNC3 antibody (0.2 ug/ml) staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot
KCNC3 antibody (0.2 ug/ml) staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot
KCNC3 antibody (0.2 ug/ml) staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot
KCNC3 antibody (0.2 ug/ml) staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Requested From:
Date Requested: 6/18/2018
Date Requested: 6/18/2018
