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Anti-JUP/CTNNG/Junction Plakoglobin Antibody (aa732-745) IHC-plus™ LS-B2204

Note: This antibody replaces LS-C34647, LS-C77814

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml Unavailable

Most Popular JUP/CTNNG/Junction Plakoglobin Antibodies

Anti-JUP/CTNNG/Junction Plakoglobin Antibody (clone PG 5.1) LS-C21268
Mouse Monoclonal [clone PG 5.1] (IgG2b) to Bovine JUP/CTNNG/Junction Plakoglobin
Bovine, Human, Mouse, Rat, Chicken
IHC, ICC, Western blot, Immunoprecipitation
Unconjugated
Anti-JUP/CTNNG/Junction Plakoglobin Antibody (clone 2G9) IHC-plus™ LS-B4364
Mouse Monoclonal [clone 2G9] (IgG1,k) to Human JUP/CTNNG/Junction Plakoglobin
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-JUP/CTNNG/Junction Plakoglobin Antibody (C-Terminus) IHC-plus™ LS-B5281
Rabbit Polyclonal (IgG) to Human JUP/CTNNG/Junction Plakoglobin
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human JUP/CTNNG/Junction Plakoglobin
Human, Monkey, Mouse, Dog, Hamster, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human JUP/CTNNG/Junction Plakoglobin
Goat
Human, Monkey, Mouse, Dog, Hamster, Rabbit (tested or 100% immunogen sequence identity)
Rat, Bat, Bovine, Pig (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • ELISA (1:32000)

Specificity and Use

JUP/CTNNG/Junction Plakoglobin antibody was raised against synthetic peptide C-GLRPPYPTADHMLA from the C-terminus of human JUP / Gamma Catenin (NP_002221.1, NP_068831.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Dog, Hamster, Panda, Rabbit (100%); Rat, Bat, Bovine, Pig, Opossum (93%); Elephant (86%).
LS-E25379 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2204. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa732-745
Human JUP / Gamma Catenin. This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1.
Immunohistochemistry: LS-B2204 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2204 was determined to be 5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About JUP/CTNNG/Junction Plakoglobin

P14923 NM_002230 NP_002221.1

JUP Antibody, Desmoplakin-3 Antibody, CTNNG Antibody, DPIII Antibody, Gamma catenin Antibody, Junction plakoglobin Antibody, PKGB Antibody, ARVD12 Antibody, Catenin gamma Antibody, Desmoplakin III Antibody, DP3 Antibody, PDGB Antibody

Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques.

Immunohistochemistry

Immunohistochemistry
Anti-Gamma Catenin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2204 concentration 5 ug/ml.

Western blot

Western blot
EB05500 staining (1µg/ml) of Rat Eye lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot

Western blot
EB05500 (0.3µg/ml) staining of A431 (A) and NIH3T3 (B) cell lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
 

Requested From: 
Date Requested: 3/24/2017

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