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Anti-ITGB5 / Integrin Beta 5 Antibody (Extracellular Domain) LS-C36943

Ordering

Wt. Vol. Conc. Price
100 µg 500 µl 0.2 mg/ml $650
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular ITGB5 / Integrin Beta 5 Antibodies

Anti-ITGB5 / Integrin Beta 5 Antibody (clone 2C4) LS-C197557
Mouse Monoclonal [clone 2C4] (IgG1,k) to Human ITGB5 / Integrin Beta 5
Human
Western blot, ELISA, RNA interference
Unconjugated
Western blot Image
Anti-ITGB5 / Integrin Beta 5 Antibody (aa215-244, APC) LS-C260520
Rabbit Polyclonal (IgG) to Human ITGB5 / Integrin Beta 5
Human
IHC, Western blot, ELISA
APC Conjugated
Anti-ITGB5 / Integrin Beta 5 Antibody (aa216-244) IHC-plus™ LS-B10667
Rabbit Polyclonal to Human ITGB5 / Integrin Beta 5
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (2)
Sheep Polyclonal (IgG) to Human ITGB5 / Integrin Beta 5
Human, Mouse, Dog
ICC, Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human ITGB5 / Integrin Beta 5
Sheep
Human, Mouse, Dog (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • ICC (10 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • Flow Cytometry
  • ELISA (0.5 - 1 µg/ml)

Specificity and Use

ITGB5 / Integrin Beta 5 antibody was raised against cHO cell-derived rhIntegrin b5 extracellular domain.
Extracellular Domain
Recognizes human Integrin b5 extracellular domain. In Direct ELISAs and Western Blots, this antibody shows less than 5% cross-reactivity with rhIntegrin b1, rhIntegrin b3, rhIntegrin b4, rhIntegrin b6, and rmIntegrin b7. Species sequence Homology: mouse, 91%, canine, 89%.
Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and ELISA. Western Blot: 0.1-0.2 ug/ml. Detection limit ~5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: Dilute antibody to 50 ug/ml and add 10 ul of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibodies may be visualized by adding 10 ul of a 25 ug/ml stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome. Immunocytochemistry: 10 ug/ml to detect b5 in MG-63 human osteosarcoma cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine Red coupled anti-sheep IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: 0.5-1.0 ug/ml. Detection limit ~0.2 ng/well.

Packaging

Lyophilized, PBS, 5% trehalose
500 µl Sterile PBS
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (2)

1
Integrin-modulating therapy prevents fibrosis and autoimmunity in mouse models of scleroderma. Gerber EE, Gallo EM, Fontana SC, Davis EC, Wigley FM, Huso DL, Dietz HC. Nature. 2013 Nov;503:126-30. [Full Text Article] [PubMed:24107997] [PMC:PMC3992987]
2
alpha V-integrins are required for mechanotransduction in MDCK epithelial cells. Ter[Character e4]v[Character e4]inen TP, Myllym[Character e4]ki SM, Friedrichs J, Strohmeyer N, Moyano JV, Wu C, Matlin KS, Muller DJ, Manninen A. PloS one. 2013 8:e71485. [Full Text Article] [PubMed:23977051] [PMC:PMC3747215]

About ITGB5 / Integrin Beta 5

P18084 NM_002213 NP_002204.2

ITGB5 Antibody, Integrin Beta 5 Antibody, Integrin beta-5 Antibody, Integrin beta-5 subunit Antibody, Integrin, beta 5 Antibody

Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.

Requested From: United States
Date Requested: 12/4/2016

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