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Anti-ITGAE / CD103 Antibody (clone AX.14) LS-C140406

Catalog Size Price
LS-C140406-100 100 µg Unavailable

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100% Guaranteed
Mouse Monoclonal [clone AX.14] (IgG1) to Human ITGAE / CD103
Human
Flow Cytometry
Unconjugated

Details

Human ITGAE / CD103
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [AX.14]
Unconjugated
Protein A/G purified
Unmodified

Applications

Flow Cytometry

Specificity and Use

Human ITGAE / CD103
PBMC: Add 10 ul of antibody/10^6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2 to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.

Packaging

PBS, 0.08% Sodium Azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About ITGAE / CD103

P38570 NM_002208 NP_002199.3

ITGAE Antibody, CD103 Antibody, CD103 antigen Antibody, Integrin alpha-E Antibody, HML-1 antigen Antibody, Integrin alpha-IEL Antibody, Alpha polypeptide) Antibody, Mucosal lymphocyte 1 antigen Antibody, HUMINAE Antibody

Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, this protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen.

Requested From: 
Date Requested: 8/23/2017

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