Target
Human ITGAE / CD103
Synonyms
ITGAE | CD103 | CD103 antigen | Integrin alpha-E | HML-1 antigen | Integrin alpha-IEL | Alpha polypeptide) | Mucosal lymphocyte 1 antigen | HUMINAE
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
FITC,
PE.
Purification
Protein A/G purified
Specificity
Human ITGAE / CD103
Usage
PBMC: Add 10 ul of antibody/10^6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2 to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.
Presentation
PBS, 0.08% Sodium Azide
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee