Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
IHC - Frozen (1:100 - 1:800)
Specificity and Use
The antigen is CD49b, the epitope has not been further characterized. Antigen distribution: Isolated cells: The antibody is positive on the following melanoma cell lines: BRO, SK-Mel-13, SK-Mel-19, SK-Mel-29, SK-Mel-113, and negative on normal monocytes. Tissue sections: Acetone fixed tissue sections of melanoma larger than 1.5mm show positive staining whereas the majority of benign melanocytic nevi (>95%) are negative. Specificity: Human: CD49b. Other: not tested.
Lyophilized from PBS pH 7.2, 10mg/ml BSA, 0.01% thimerosal
ITGA2 encodes integrin alpha chain 2. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing alpha integrin 2 combines with beta integrin 1 to form a collagen-binding integrin referred to as glycoprotein Ia/IIa when expressed on platelets, and very late (activation) antigen 2 ('VLA-2') when found on T-cells. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.