IRS1 antibody LS-C8274 is an unconjugated rabbit polyclonal antibody to IRS1 from human, mouse, rat and other species. Validated for ELISA and WB.
Human, Monkey, Mouse, Rat, Dog, Horse, Rabbit
(tested or 100% immunogen sequence identity)
Bovine, Pig, Chicken (at least 90% immunogen sequence identity)
- Western blot (1:250 - 1:1500)
- ELISA (1:2000 - 1:4000)
IRS1 antibody was raised against synthetic peptide corresponding to aa 298-316 of Human IRS-1 at 180kD. Peptide Sequence: GLTRRSRTE-pS-ITATSPASM.
Recognizes human IRS-1, pS307. Minimal reactivity with non phosphorylated Ser307. Species sequence homology: mouse, rat, canine, vervet monkey 100%, porcine, bovine -94%, chicken 88%.
Suitable for use in ELISA and Western Blot. ELISA: 1:2000-1:4000; Assayed against 0.1 ug of the immunizing peptide. Western Blot: 1:250-1:1500.
PBS, pH 7.2, 0.01% Sodium Azide, No stabilizing proteins added
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.