Human, Monkey, Mouse, Rat, Dog, Horse, Rabbit
(tested or 100% immunogen sequence identity)
Bovine, Pig, Chicken (at least 90% immunogen sequence identity)
- Western blot (1:250 - 1:1500)
- ELISA (1:2000 - 1:4000)
IRS1 antibody was raised against synthetic peptide corresponding to aa 298-316 of Human IRS-1 at 180kD. Peptide Sequence: GLTRRSRTE-pS-ITATSPASM.
Recognizes human IRS-1, pS307. Minimal reactivity with non phosphorylated Ser307. Species sequence homology: mouse, rat, canine, vervet monkey 100%, porcine, bovine -94%, chicken 88%.
Suitable for use in ELISA and Western Blot. ELISA: 1:2000-1:4000; Assayed against 0.1 ug of the immunizing peptide. Western Blot: 1:250-1:1500.
PBS, pH 7.2, 0.01% Sodium Azide, No stabilizing proteins added
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.