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Anti-IRS1 Antibody (phospho-Ser307) LS-C18824

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IRS1 Antibodies

Anti-IRS1 Antibody (Internal) IHC-plus™ LS-B1373
Rabbit Polyclonal to Human IRS1
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-IRS1 Antibody (aa431-446) LS-C125829
Mouse Monoclonal (IgG2a,k) to Human IRS1
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig
ICC, Western blot, Immunoprecipitation
Unconjugated
Anti-IRS1 Antibody (phospho-Ser636) LS-C178019
Rabbit Polyclonal to Human IRS1
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human IRS1
Human
Western blot, ELISA
Unconjugated

Details

Human IRS1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:250 - 1:1500)
  • ELISA (1:2000 - 1:10000)

Specificity and Use

IRS1 antibody was raised against synthetic peptide from human IRS1.
pSer307
Amino acids 298-316 of human IRS1 protein.
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 180 kD in size corresponding to phosphorylated IRS1 protein by western blotting in the appropriate cell lysate or extract. Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS307 of IRS1 protein.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About IRS1

P35568 NM_005544 NP_005535.1

IRS1 Antibody, IRS-1 Antibody, HIRS-1 Antibody, Insulin receptor substrate 1 Antibody

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.

Western blot

Western blot
Anti-IRS1 pS307 Antibody - Western Blot. Western blot of Affinity Purified anti-IRS1 pS307 antibody shows detection of a band at ~180 kD believed to represent phosphorylated IRS1 (arrowhead). Lane 1 shows staining of human 293 cell lysate. Lane 2 shows staining of 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/ml of anisomysin for 30 min. The pronounced staining of the band at 180 kD is not seen when the antibody was pre-incubated with immunizing peptide prior to reaction (data not shown). The identity of the intensely reactive bands at ~70 kD in both lane 1 and 2 is unknown, although these bands were also competed out by pre-incubation with the immunizing peptide. Approximately 25 ug of each lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum, 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:250. Reaction occurred overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
 

Requested From: United States
Date Requested: 12/2/2016

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