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Anti-IRF7 Antibody (aa1-150, clone 3D9) IHC-plus™ LS-B2945

Note: This antibody replaces LS-C93757, LS-C70830, LS-C130132

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IRF7 Antibodies

Anti-IRF7 Antibody (C-Terminus) IHC-plus™ LS-B577
Rabbit Polyclonal to Human IRF7
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-IRF7 Antibody (aa1-150, clone 3D9) IHC-plus™ LS-B2945
Mouse Monoclonal [clone 3D9] (IgG1,k) to Human IRF7
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Mouse Monoclonal [clone 3D9] (IgG1,k) to Human IRF7
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human IRF7
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [3D9]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (20 µg/ml)
  • Western blot (1:1000 - 1:2000)
  • ELISA

Specificity and Use

IRF7 antibody was raised against recombinant human IRF7 (1-150aa) purified from E. coli.
aa1-150
Immunohistochemistry: LS-B2945 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2945 was determined to be 20 ug/ml.

Packaging

PBS, pH 7.4, 0.1% sodium azide. Sourced in Ascites.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

Publications (1)

1
IRF6 is a mediator of Notch pro-differentiation and tumour suppressive function in keratinocytes. Restivo G, Nguyen BC, Dziunycz P, Ristorcelli E, Ryan RJ, [Character d6]zuysal, Di Piazza M, Radtke F, Dixon MJ, Hofbauer GF, Lefort K, Dotto GP. The EMBO journal. 2011 Nov;30:4571-85. [Full Text Article] [PubMed:21909072] [PMC:PMC3243593]

About IRF7

Q92985 NM_004031 NP_004022.2

IRF7 Antibody, Interferon regulatory factor 7 Antibody, IRF-7 Antibody, IRF7A Antibody, IRF-7H Antibody

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters.

Immunohistochemistry

Immunohistochemistry
Anti-IRF7 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2945 concentration 20 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-IRF7 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2945 concentration 20 ug/ml.

Western blot

Western blot
Cell lysates of Jurkat (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IRF7 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
 

Requested From: United States
Date Requested: 12/10/2016

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