LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-IRAK1 / IRAK Antibody (clone 3A9) IHC-plus™ LS-B4361

Note: This antibody replaces LS-C104979

Ordering

Wt. Vol. Conc. Price
50 µg - - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IRAK1 / IRAK Antibodies

Anti-IRAK1 / IRAK Antibody (clone 3F7) IHC-plus™ LS-B4360
Mouse Monoclonal [clone 3F7] (IgG2a,k) to Human IRAK1 / IRAK
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IRAK1 / IRAK Antibody (aa405-433) LS-C164426
Rabbit Polyclonal to Mouse IRAK1 / IRAK
Mouse
Western blot, Flow Cytometry
Unconjugated
Western blot Image
Anti-IRAK1 / IRAK Antibody (aa530-693, Biotin) LS-C225689
Mouse Monoclonal (IgG2a) to Human IRAK1 / IRAK
Human
Immunofluorescence, Western blot, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 3A9] (IgG1,k) to Human IRAK1 / IRAK
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA, RNA interference
Unconjugated

Details

Human IRAK1 / IRAK
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [3A9]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • Immunoprecipitation
  • ELISA
  • RNA interference

Specificity and Use

IRAK1 / IRAK antibody was raised against iRAK1 (AAH54000, 530 a.a. ~ 693 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Cell lysate) - positive control A-431. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein). RNAi Knockdown (Antibody validated).

Packaging

PBS, pH 7.2
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About IRAK1 / IRAK

P51617 NM_001569 NP_001560.2

IRAK1 Antibody, IRAK-1 Antibody, Pelle Antibody, Il1rak Antibody, IRAK Antibody, Pelle homolog Antibody

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation.

Immunohistochemistry

Immunohistochemistry
Anti-IRAK1 / IRAK antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4361 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to IRAK1 on HeLa cell (antibody concentration 10 ug/ml).

Western blot

Western blot
Western blot of IRAK1 expression in transfected 293T cell line by IRAK1 monoclonal antibody clone 3A9.

Western blot

Western blot
IRAK1 monoclonal antibody clone 3A9 Western blot of IRAK1 expression in A-431.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged IRAK1 is approximately 0.03 ng/ml as a capture antibody.

RNA interference

RNA interference
WB analysis of IRAK1 over-expressed 293 cell line, cotransfected with IRAK1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with IRAK1 MAb clone 3A9. GAPDH ( 36.1 kD ) used as specificity and loading control.

Requested From: United States
Date Requested: 12/9/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number