LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
IQGAP1 Antibody (aa863‑1657) LS‑C8229
IQGAP1 antibody LS-C8229 is an unconjugated mouse monoclonal antibody to human IQGAP1. Validated for IP and WB.
Antibody
Catalog
Size
Price
LS-C8229-200
200 µg
$650

Popular IQGAP1 Products

Immunoperoxidase of monoclonal antibody to IQGAP1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 1 ug/ml]
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Antibody
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA
Detection of Human IQGAP1 by Immunohistochemistry and Immunofluorescence. Sample: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-IQGAP1 used at a dilution of 1:1000 (0.2 ug/ml) and 1:200 (1 ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100.
Species: Human, Dog
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Proximity Ligation Assay
Detection of Human IQGAP1 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-IQGAP1 used at a dilution of 1:100.
Species: Human, Dog
Applications: IHC, IHC - Paraffin, Immunofluorescence

Product Description

IQGAP1 antibody LS-C8229 is an unconjugated mouse monoclonal antibody to human IQGAP1. Validated for IP and WB.

Specifications

Target
Human IQGAP1
Synonyms
IQGAP1, HUMORFA01, p195, KIAA0051, RasGAP-like with IQ motifs, SAR1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 2 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
IQGAP1 antibody was raised against a Glu-Glu tagged C-terminal fragment corresponding to aa863-1657 of human IQGAP1 purified from baculovirus infected insect cells.
Epitope
aa863-1657
Specificity
Recognizes human IQGAP1 at 185kD. Does not recognize IQGAP2.
Usage
Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug immunoprecipitated IQGAP1 from 500 ug of a HepG2 RIPA lysate.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About IQGAP1
Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth. P46940 NM_003870 NP_003861.1


Publications (0)


Customer Reviews (0)


Requested From: United States
Date Requested: 10/16/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy