IQGAP1 antibody LS-C8229 is an unconjugated mouse monoclonal antibody to human IQGAP1. Validated for IP and WB.
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot (0.5 - 2 µg/ml)
- (applications tested for the base form of this product only)
IQGAP1 antibody was raised against a Glu-Glu tagged C-terminal fragment corresponding to aa863-1657 of human IQGAP1 purified from baculovirus infected insect cells.
Recognizes human IQGAP1 at 185kD. Does not recognize IQGAP2.
Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug immunoprecipitated IQGAP1 from 500 ug of a HepG2 RIPA lysate.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.