Usage
Suitable for use in Flow Cytometry, Immunoprecipitation and Function Blocking. Not suitable for use in Western Blot and Immunohistochemistry. Flow Cytometry (effective for mouse and human): Use buffers containing 1 mM Mg, since the epitope recognized is divalent cation-dependent. Immunoprecipitation: Effective. Protein G or rabbit anti-mouse IgG recommended for capture. Function blocking: Inhibition of alpha V beta 6-mediated cell adhesion and cell migration are achieved at a concentration of 100 ug/ml using beta 6 transfected cells tested against TGFbeta LAP. Note: Cell lines adhere to simple ligands (e. g. fibronectin) using more integrins than alphav/beta6, so it would be necessary to also block other integrins (e. g. with anti-beta1 antibody) to see any effect of this antibody on adhesion to fibronectin. For example, H441 cells, which naturally express high levels of alphaV/beta6, may be used as a positive control.
Presentation
PBS, No preservatives added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee