Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal (IgG2a) to Rat Integrin Alpha1 Beta1
IHC - Frozen, Immunoprecipitation, Flow Cytometry
Rat Integrin Alpha1 Beta1
Rat (tested or 100% immunogen sequence identity)
IHC - Frozen
Specificity and Use
Rat pheochromocytoma PC12 cells with myeloma P3X63ag9.653 cells.
Antibody MAB2513 is directed against rat integrin a1b1 (VLA-1), a receptor for collagen and laminin. Antibody specificity has been confirmed by 1) its ability to immunoprecipitate beta1 (Mr ~200 kD) and b1 (Mr ~120 kD) from (125)I-surface labeled PC12 cells and 2) reciprocal depletion of VLA-1 antigen from cell lysate with antiserum against the cytoplasmic domain of a1 integrin subunit. Analysis of immunoprecipitation by SDS-PAGE was carried out under non-reducing conditions using a 6% gel. The antibody will not recognize the individual integrin subunits, only the VLA-1 complex. The antibody is not effective in western blots of VLA-1 for this reason, only immunoprecipitation or staining (acetone fixed only)
Immunoprecipitation of VLA-1 integrin from detergent cell lysates. Suggested dilution: 2 g per 10E6 cell equivalent. Flow cytometry: 10 ug/ml. Immunohistochemistry: Detects VLA-1 in acetone-fixed frozen tissue sections from gut, heart, and lungs. Suggesti
0.02 M phosphate buffer, pH 7.6, 0.25 M sodium chloride, 0.1% sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.