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Anti-ING4 Antibody (aa164-175) IHC-plus™ LS-B386

Note: This antibody replaces LS-C19021


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular ING4 Antibodies

Anti-ING4 Antibody (aa164-175) IHC-plus™ LS-B386
Goat Polyclonal to Human ING4
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Immunohistochemistry Image
Anti-ING4 Antibody (aa110-159) IHC-plus™ LS-B5821
Rabbit Polyclonal (IgG) to Human ING4
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Rabbit
IHC - Paraffin, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human ING4
IHC - Paraffin, Immunofluorescence, Western blot, ELISA


Human ING4
Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1:500 - 1:3000)
  • ELISA (1:3000 - 1:12000)

Specificity and Use

ING4 antibody was raised against synthetic peptide from human ING4.
aa 164-175 of Human p29 ING4 protein (Inhibitor of growth family, member 4). This sequence only shows homology to isoform 1 for ING4.
Immunohistochemistry: LS-B386 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B386 was determined to be 2.5 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About ING4

Q9UNL4 NM_016162 NP_057246.2

ING4 Antibody, Brain my036 protein Antibody, Inhibitor of growth protein 4 Antibody, My036 Antibody, p29ING4 Antibody

Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may function in DNA replication. May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation.


Anti-ING4 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B386 concentration 5 ug/ml.

Western blot

Western blot
Anti-p29 ING4 Polyclonal Antibody - Western Blot. Anti-p29 ING4 polyclonal antibody detects ING4 protein by western blot. This antibody was used at 1.0 ug/ml to detect ING4 (lane 2) present in a U2OS whole cell lysate over expressing the protein. A control lysate (lane 3) shows no background staining. Comparison to MW markers (lane 1) indicates detection of a single band at ~29 kD corresponding to ING4. A 4-20% TRIS-glycine gradient gel was used to separate the protein by SDS-PAGE under reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking using 5% non-fat dry milk in PBS, the membrane was probed with the primary antibody overnight at 4° C followed by washes and reaction with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] (code for 45 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Western blot

Western blot
Anti-p29 ING4 Polyclonal Antibody - Western Blot. Anti-p29 ING4 polyclonal antibody detects ING4 protein by Western blot of over expressed cell lysates. This antibody was used at 1.0 ug/ml to detect ING4 expression in control (-) and transformed U2OS and HeLa cell lysates. A predominant band corresponding to p29 ING4 is only seen in lysates from transformed cells. Personnel Communication, Motoko Unoki, NCI, NIH.

Requested From: United States
Date Requested: 10/27/2016

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