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Anti-IMPDH2 Antibody (clone 1E12-B6) LS-C197533

Catalog Size Price
LS-C197533-100 100 µg Unavailable

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100% Guaranteed
Mouse Monoclonal [clone 1E12-B6] (IgG1,k) to Human IMPDH2
Western blot, Immunoprecipitation, ELISA, RNA interference


Human IMPDH2
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [1E12-B6]
Purified from ascites by Protein A


  • Western blot
  • Immunoprecipitation
  • RNA interference

Specificity and Use

IMPDH2 antibody was raised against iMPDH2 (AAH12840, aa1-514 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human IMPDH2
RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate.


PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About IMPDH2

P12268 NM_000884 NP_000875.2

IMPDH2 Antibody, IMP dehydrogenase 2 Antibody, IMP oxireductase 2 Antibody, IMPD2 Antibody, IMPDH-II Antibody, Dna binding protein rfx2 Antibody, IMPD 2 Antibody, IMPDH 2 Antibody

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.

Western blot

Western blot
Western Blot analysis of IMPDH2 expression in transfected 293T cell line by IMPDH2 monoclonal antibody (M01), clone 1E12-B6.Lane 1: IMPDH2 transfected lysate(55.8 KDa).Lane 2: Non-transfected lysate.


Immunoprecipitation of IMPDH2 transfected lysate using anti-IMPDH2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IMPDH2 rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged IMPDH2 is approximately 0.3 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot analysis of IMPDH2 over-expressed 293 cell line, cotransfected with IMPDH2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with IMPDH2 monoclonal antibody (M01), clone 1E12-B6 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Requested From: 
Date Requested: 12/17/2017

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