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Anti-IL9 Antibody LS-C154094

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL9 Antibodies

Anti-IL9 Antibody (Biotin) LS-C104797
Rabbit Polyclonal to Human IL9
Human
Western blot, ELISA, Neutralization
Biotin Conjugated
Western blot Image
Anti-IL9 Antibody (C-Terminus) LS-C201136
Rabbit Polyclonal to Human IL9
Human
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human IL9
Human
IHC, Western blot, ELISA
Unconjugated

Details

Human IL9
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Ion exchange chromatography
Unmodified

Applications

  • IHC
  • Western blot (1:1000)
  • ELISA

Specificity and Use

IL9 antibody was raised against this purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-9 protein.
This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-9 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
This purified antibody has been tested for use in ELISA and western blotting. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 15 kD in size corresponding to native human IL-9 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About IL9

P15248 NM_000590 NP_000581.1

IL9 Antibody, Cytokine P40 Antibody, IL-9 Antibody, Interleukin-9 Antibody, HP40 Antibody, p40 cytokine Antibody, Interleukin 9 Antibody, T-cell growth factor P40 Antibody, p40 Antibody

IL9 is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma.

Western blot

Western blot
Anti-IL-9 Antibody - Western Blot. anti-Human IL-9 antibody shows detection of a band ~15 kD in size corresponding to recombinant human IL-9. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (LS-C60865) secondary antibody diluted 1:40000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).
 

Requested From: United States
Date Requested: 12/6/2016

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