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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL5 Antibody LS‑C7917
IL5 antibody LS-C7917 is an unconjugated rat monoclonal antibody to IL5 from human and mouse. Validated for ELISA, Flow, IF and WB.
Antibody
Catalog
Size
Price
LS-C7917-500
500 µg
$455

Popular IL5 Products

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Applications: Western blot

Product Description

IL5 antibody LS-C7917 is an unconjugated rat monoclonal antibody to IL5 from human and mouse. Validated for ELISA, Flow, IF and WB.

Specifications

Target
Mouse IL5
Synonyms
IL5, EDF, Interleukin-5, IL-5, T-cell replacing factor, TRF
Host
Rat
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot
  • Flow Cytometry
  • ELISA
Immunogen
IL5 antibody was raised against semi-purified mouse T cell clone supernatant.
Specificity
Recognizes mouse and human Interleukin-5 (IL-5).
Usage
ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse or human IL-5 protein levels.5-7 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations.8 Recombinant mouse IL-5 or Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blot applications. For Western blotting, a concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse IL-5 under reducing conditions.
Presentation
100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About IL5
IL5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. P05113 NM_000879 NP_000870.1


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Requested From: United States
Date Requested: 11/15/2018

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