(tested or 100% immunogen sequence identity)
- Western blot
- Flow Cytometry
IL5 antibody was raised against semi-purified mouse T cell clone supernatant.
Recognizes mouse and human Interleukin-5 (IL-5).
ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse or human IL-5 protein levels.5-7 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations.8 Recombinant mouse IL-5 or Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blot applications. For Western blotting, a concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse IL-5 under reducing conditions.
100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.