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Anti-IL5 Antibody (Biotin) LS-C7908

Ordering

Wt. Vol. Conc. Price
500 µg - - $465
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Rat Monoclonal (IgG2a,k) to Human IL5
Human
Immunofluorescence, Western blot, Flow Cytometry, ELISA
Biotin Conjugated

Details

Human IL5
Rat
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal
Biotin
Purified

Applications

Immunofluorescence, Western blot, Flow Cytometry, ELISA

Specificity and Use

IL5 antibody was raised against cOS-expressed recombinant human IL-5.
Recognizes human Interleukin-5 (IL-5).
ELISA: Monoclonal antibody is useful as a capture or detection antibody in a sandwich ELISA for quantifying human IL-5 protein levels.1-3,5 Purified antibody may be paired with biotinylated antibody as the detection antibody, with purified recombinant human IL-5 as the standard. Or, conversely, biotinylated may be paired with purified antibody as the capture antibody. Immunofluorescence/Flow Cytometry: This antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations.5,6 Recombinant human IL-5, unlabeled antibody, or Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blotting applications. A concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-5 under reducing conditions.

Packaging

PBS, sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About IL5

P05113 NM_000879 NP_000870.1

IL5 Antibody, EDF Antibody, Interleukin-5 Antibody, IL-5 Antibody, T-cell replacing factor Antibody, TRF Antibody

IL5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases.

Requested From: United States
Date Requested: 9/27/2016

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