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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL5 Antibody (Biotin) LS‑C7908
IL5 antibody LS-C7908 is a biotin-conjugated rat monoclonal antibody to human IL5. Validated for ELISA, Flow, IF and WB.
Antibody
Catalog
Size
Price
LS-C7908-500
500 µg
Unavailable

Popular IL5 Products

Antibody
Species: Human
Applications: Western blot, ELISA, Functional Assay
Antibody
Species: Human
Applications: Western blot, ELISA
Western Blot (reducing) of IL-5 antibody LS-C104699.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Western blot, ELISA, Neutralization
Antibody
Species: Monkey
Applications: Western blot
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

IL5 antibody LS-C7908 is a biotin-conjugated rat monoclonal antibody to human IL5. Validated for ELISA, Flow, IF and WB.
About IL5
IL5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. P05113 NM_000879 NP_000870.1

IL5 Antibody, EDF Antibody, Interleukin-5 Antibody, IL-5 Antibody, T-cell replacing factor Antibody, TRF Antibody


Specifications

Target
Human IL5
Host
Rat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Conjugations
Biotin
Purification
Purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot
  • Flow Cytometry
  • ELISA
Immunogen
IL5 antibody was raised against cOS-expressed recombinant human IL-5.
Specificity
Recognizes human Interleukin-5 (IL-5).
Usage
ELISA: Monoclonal antibody is useful as a capture or detection antibody in a sandwich ELISA for quantifying human IL-5 protein levels.1-3,5 Purified antibody may be paired with biotinylated antibody as the detection antibody, with purified recombinant human IL-5 as the standard. Or, conversely, biotinylated may be paired with purified antibody as the capture antibody. Immunofluorescence/Flow Cytometry: This antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations.5,6 Recombinant human IL-5, unlabeled antibody, or Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blotting applications. A concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-5 under reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, sodium azide.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 7/20/2018

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