Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
IL5 antibody was raised against cOS-expressed recombinant human IL-5.
Recognizes human Interleukin-5 (IL-5).
ELISA: Monoclonal antibody is useful as a capture or detection antibody in a sandwich ELISA for quantifying human IL-5 protein levels.1-3,5 Purified antibody may be paired with biotinylated antibody as the detection antibody, with purified recombinant human IL-5 as the standard. Or, conversely, biotinylated may be paired with purified antibody as the capture antibody. Immunofluorescence/Flow Cytometry: This antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations.5,6 Recombinant human IL-5, unlabeled antibody, or Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blotting applications. A concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-5 under reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
IL5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases.