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Anti-IL5 Antibody (Azide-free) LS-C7918

Ordering

Wt. Vol. Conc. Price
500 µg - - $455
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL5 Antibodies

Anti-IL5 Antibody LS-C104699
Rabbit Polyclonal to Human IL5
Human
IHC, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal (IgG1,k) to Mouse IL5
Mouse, Human
Immunofluorescence, Western blot, Flow Cytometry, ELISA, Neutralization
Unconjugated, Azide-free

Details

Mouse IL5
Rat
Mouse, Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Unconjugated
Purified
Azide-free

Applications

  • Immunofluorescence
  • Western blot
  • Flow Cytometry
  • ELISA
  • Neutralization

Specificity and Use

IL5 antibody was raised against semi-purified mouse T cell clone supernatant.
Recognizes mouse and human Interleukin-5 (IL-5).
ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse or human IL-5 protein levels.5-7 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations.8 Recombinant mouse IL-5 or Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blot applications. For Western blotting, a concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse IL-5 under reducing conditions. Neutralization: The LE/AF form can be used for neutralizing mouse IL-5 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Packaging

PBS. No preservative added.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About IL5

P05113 NM_000879 NP_000870.1

IL5 Antibody, EDF Antibody, Interleukin-5 Antibody, IL-5 Antibody, T-cell replacing factor Antibody, TRF Antibody

IL5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases.

Requested From: United States
Date Requested: 12/8/2016

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