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Anti-IL3 Antibody LS-C154090

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL3 Antibodies

Anti-IL3 Antibody (clone MP2-8F8, Azide-free, Low Endotoxin) LS-C149564
Rat Monoclonal [clone MP2-8F8] (IgG1,k) to Mouse IL3
Mouse
Neutralization
Unconjugated, Azide-free, Low Endotoxin

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human IL3
Human
Western blot, ELISA
Unconjugated

Details

Human IL3
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Ion exchange chromatography
Unmodified

Applications

  • Western blot (1:1000)
  • ELISA

Specificity and Use

IL3 antibody was raised against iL-3 antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-3 protein.
This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-3 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 15 kD in size corresponding to native human IL-3 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About IL3

P08700 NM_000588 NP_000579.2

IL3 Antibody, Hematopoietic growth factor Antibody, IL-3 Antibody, Mast-cell growth factor Antibody, MULTI-CSF Antibody, P-cell-stimulating factor Antibody, Interleukin-3 Antibody, Mast cell growth factor Antibody, MCGF Antibody, P-cell stimulating factor Antibody

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Western blot

Western blot
Anti-IL-3 Antibody - Western blot. anti-Human IL-3 antibody shows detection of a band ~15 kD in size corresponding to recombinant human IL-3. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (LS-C60865) secondary antibody diluted 1:40000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).
 

Requested From: United States
Date Requested: 12/6/2016

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