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Anti-IL25 / IL17E Antibody LS-C71025

Ordering

Wt. Vol. Conc. Price
500 µg 500 µl 1 mg/ml $650
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL25 / IL17E Antibodies

Anti-IL25 / IL17E Antibody (aa115-132, clone 68C1039.2) IHC-plus™ LS-B123
Mouse Monoclonal [clone 68C1039.2] (IgG1) to Human IL25 / IL17E
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-IL25 / IL17E Antibody (PE) LS-C71027
Rat Monoclonal (IgG2b) to Mouse IL25 / IL17E
Mouse
Flow Cytometry
PE Conjugated
Flow Cytometry Image
Anti-IL25 / IL17E Antibody (PE) LS-C37048
Mouse Monoclonal (IgG1) to Human IL25 / IL17E
Human
Flow Cytometry
PE Conjugated

100% Guaranteed 100% Guaranteed
Rat Monoclonal (IgG2b) to Mouse IL25 / IL17E
Mouse
Flow Cytometry, ELISA
Unconjugated

Details

Mouse IL25 / IL17E
Rat
Mouse (tested or 100% immunogen sequence identity)
IgG2b Monoclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • Flow Cytometry
  • ELISA

Specificity and Use

IL25 / IL17E antibody was raised against e. coli-derived rmIL-17E.
Recognizes mouse IL-17E. In sandwich ELISAs no cross-reactivity or interference was observed with rhIL-17E, rmIL-17, rmIL-17B, C, D, or F.
Suitable for use in ELISA and Flow Cytometry. ELISA (Capture): 2-8 ug/m. Can be used as a capture reagent in a sandwich immunoassay in combination with a biotinylated, mouse IL-17E antibody and recombinant mouse IL-17E, as the standard. Flow Cytometry: For intracellular flow cytometry to detect IL-17E, cells must first be fixed and permeabilized with 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 25 ug/ml and add 10 ul of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibody may be visualized by adding a secondary developing reagent such as anti-rat IgG conjugated to a fluorochrome.

Packaging

Lyophilized from PBS, 5% trehalose.
500 µl 40-50% glycerol, PBS.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About IL25 / IL17E

Q9H293 NM_022789 NP_073626.1

IL25 Antibody, Interleukin 17E Antibody, Interleukin 25 Antibody, Interleukin-25 Antibody, IL17E Antibody, IL-17E Antibody, IL-25 Antibody, Interleukin-17E Antibody

The IL17 proteins are a family of potent cytokines that act to induce proinflammatory responses. Studies have shown that IL17E binds strongly to IL17RB. Receptor binding of ligand has been shown to lead to the activation of nuclear factor kappa-B and production of IL8.

Requested From: United States
Date Requested: 12/10/2016

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