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Anti-IL2 Antibody IHC-plus™ LS-B7472

Note: This antibody replaces LS-C104730

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human IL2
Human
IHC - Paraffin, Western blot, ELISA, Neutralization, Sandwich ELISA
Unconjugated

Details

Human IL2
Goat
Human (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Western blot, ELISA, Neutralization (0.12 - 0.16 µg/ml), Sandwich ELISA

Specificity and Use

IL2 antibody was raised against e.coli derived recombinant Human IL-2.
Human IL-2
Immunohistochemistry: LS-B7472 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-2 (2 ng/ml), a concentration of 0.12-0.16 ug/ml of this antibody is required. ELISA: To detect hIL-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-2 (LS-C104359) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-2. Western Blot: To detect hIL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About IL2

P60568 NM_000586 NP_000577.2

IL2 Antibody, Aldesleukin Antibody, Interleukin 2 Antibody, Lymphokine Antibody, IL-2 Antibody, Interleukin-2 Antibody, TCGF Antibody, T cell growth factor Antibody, T-cell growth factor Antibody


Immunohistochemistry

Immunohistochemistry
Anti-IL-2 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7472 concentration 5 ug/ml.

Western blot

Western blot
Western Blot (reducing) of IL-2 antibody LS-B7472

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of IL-2 antibody LS-B7472
 

Requested From: United States
Date Requested: 4/26/2015

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