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IL2 Antibody (HRP) LS‑C153807
IL2 antibody LS-C153807 is an HRP-conjugated rabbit polyclonal antibody to human IL2. Validated for ELISA, IHC and WB.
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Species:
Chicken
Applications:
ELISA, Functional Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA, Neutralization, Sandwich ELISA
Product Description
IL2 antibody LS-C153807 is an HRP-conjugated rabbit polyclonal antibody to human IL2. Validated for ELISA, IHC and WB.
About IL2
IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene.
P60568
NM_000586
NP_000577.2
IL2 Antibody, Aldesleukin Antibody, Interleukin 2 Antibody, Lymphokine Antibody, IL-2 Antibody, Interleukin-2 Antibody, TCGF Antibody, T cell growth factor Antibody, T-cell growth factor Antibody
Specifications
Target
Human IL2
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
- IHC (1:500 - 1:2500)
- Western blot (1:1000 - 1:5000)
- ELISA
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Immunogen
IL2 antibody was raised against this purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-2 protein.
Specificity
This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-2 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Usage
This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 15 kD in size corresponding to human IL-2 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% Gentamicin sulfate
Reconstitution
100 µl deionized water
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Western blot
IL-2 Antibody Peroxidase Conjugated Western Blot. Western Blot of Rabbit anti-IL-2 Antibody Peroxidase Conjugated Lane 1: Human IL-2 Recombinant Protein Load: 50 ng per lane Secondary antibody: Peroxidase Conjugated IL-2 Antibody at 1:1000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 16 kD, 16 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
IL-2 Antibody Peroxidase Conjugated Western Blot. Western Blot of Rabbit anti-IL-2 Antibody Peroxidase Conjugated Lane 1: Human IL-2 Recombinant Protein Load: 50 ng per lane Secondary antibody: Peroxidase Conjugated IL-2 Antibody at 1:1000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 16 kD, 16 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
IL-2 Antibody Peroxidase Conjugated Western Blot. Western Blot of Rabbit anti-IL-2 Antibody Peroxidase Conjugated Lane 1: Human IL-2 Recombinant Protein Load: 50 ng per lane Secondary antibody: Peroxidase Conjugated IL-2 Antibody at 1:1000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 16 kD, 16 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
IL-2 Antibody Peroxidase Conjugated Western Blot. Western Blot of Rabbit anti-IL-2 Antibody Peroxidase Conjugated Lane 1: Human IL-2 Recombinant Protein Load: 50 ng per lane Secondary antibody: Peroxidase Conjugated IL-2 Antibody at 1:1000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 16 kD, 16 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From:
Date Requested: 6/25/2018
Date Requested: 6/25/2018
