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Anti-IL2 Antibody LS-C154093


Wt. Vol. Conc. Price
100 µg 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular IL2 Antibodies

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Western blot Image
Anti-IL2 Antibody IHC-plus™ LS-B7472
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IHC - Paraffin, Western blot, ELISA, Neutralization, Sandwich ELISA
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100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human IL2
Western blot, ELISA


Human IL2
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Ion exchange chromatography


  • Western blot (1:1000)

Specificity and Use

IL2 antibody was raised against iL-2 antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-2 protein.
This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-2 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 15 kD in size corresponding to native human IL-2 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About IL2

P60568 NM_000586 NP_000577.2

IL2 Antibody, Aldesleukin Antibody, Interleukin 2 Antibody, Lymphokine Antibody, IL-2 Antibody, Interleukin-2 Antibody, TCGF Antibody, T cell growth factor Antibody, T-cell growth factor Antibody

IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene.

Western blot

Western blot
Anti-IL-2 Antibody - Western Blot. anti-Human IL-2 antibody shows detection of a band ~15 kD in size corresponding to recombinant human IL-2. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (LS-C60865) secondary antibody diluted 1:40000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).

Requested From: United States
Date Requested: 10/24/2016

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